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Improvement of Strain Penicillium sp. EZ-ZH190 for Tannase Production by Induced Mutation

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Abstract

In the search for an efficient producer of tannase, Penicillium sp. EZ-ZH190 was subjected to mutagenesis using heat treatment and strain EZ-ZH290 was isolated. The maximum tannase in this mutant strain was 4.32 U/mL with an incubation period of 84 h as compared to wild strain EZ-ZH190 where the incubation period was 96 h with a maximum enzyme activity of 4.33 U/mL. Also, the Penicillium sp. EZ-ZH290 tannase had a maximum activity at 40 °C and pH 5.5. Then, the spores of strain EZ-ZH290 were subjected to γ irradiation mutagenesis and strain EZ-ZH390 was isolated. Strain EZ-ZH390 exhibited higher tannase activity (7.66 U/mL) than the parent strain EZ-ZH290. It was also found that Penicillium sp. EZ-ZH390 tannase had an optimum activity at 35 °C and a broad pH profile with an optimum at pH 5.5. The tannase pH stability of Penicillium sp. EZ-ZH390 and its maximum production of tannase followed the same trend for five generations confirming the occurrence of stable mutant. This paper is shown that γ irradiation can mutate the Penicillium sp. leading to increase the tannase production.

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References

  1. Pepi, M., Lampariello, L. R., & Altieri, R. (2010). International Biodeterioration and Biodegradation, 64, 73–80. doi:10.1016/j.ibiod.2009.10.009.

    Article  CAS  Google Scholar 

  2. Frutos, P., Hervas, G., Giraldez, F. J., & Mantec, A. R. (2004). Spanish Journal of Agricultural Research, 2, 191–202.

    Google Scholar 

  3. Bele, A. A., Jadhav, V. M., & Kadam, V. J. (2010). Asian Journal of Plant Sciences, 9, 209–214. doi:10.3923/ajps.2010.209.214.

    Article  Google Scholar 

  4. Belmares, R., Contreras-Esquivel, J. C., Rodriguez-Herrera, R., Coronel, A. R., & Aguilar, C. N. (2004). LWT—Food Science and Technology, 37, 857–864. doi:10.1016/j.lwt.2004.04.002.

    CAS  Google Scholar 

  5. Mingshu, L., Kai, Y., Qiang, H., & Dongying, J. (2006). Journal of Basic Microbiology, 46, 68–84. doi:10.1002/jobm.200510600.

    Article  Google Scholar 

  6. Lekha, P. K., & Lonsane, B. K. (1997). Advances in Applied Microbiology, 44, 215–260. doi:10.1016/S0065-2164(08)70463-5.

    Article  CAS  Google Scholar 

  7. Rodriguez, H., Curiel, J. A., Landete, J. M., et al. (2009). International Journal of Food Microbiology, 132, 79–90. doi:10.1016/j.ijfoodmicro.2009.03.025.

    Article  CAS  Google Scholar 

  8. Ramirez, L., Arrizon, J., Sandoval, G., et al. (2008). Applied Biochemistry and Biotechnology, 151, 711–723. doi:10.1007/s12010-008-8319-8.

    Article  CAS  Google Scholar 

  9. Rodriguez-Duran, L. V., Valdivia-Urdiales, B., Contreras-Esquivel, J. C., Rodriguez-Herrera, R., & Aguilar, C. N. (2011). Enzyme Research, 1–20, doi: 10.4061/2011/823619 .

  10. Aguilar, C. N., Rodriguez, R., Gutierrez-Sanchez, G., et al. (2007). Applied Microbiology and Biotechnology, 76, 47–59. doi:10.1007/s00253-007-1000-2.

    Article  CAS  Google Scholar 

  11. Aguilar, C. N., & Gutierrez-Sanchez, G. (2001). Food Science and Technology International, 7, 373–382. doi:10.1106/69M3-B30K-CF7Q-RJ5G.

    CAS  Google Scholar 

  12. Belur, P. D., & Mugeraya, G. (2011). Research Journal of Microbiology, 6, 25–40.

    Article  CAS  Google Scholar 

  13. Cruz-Hernandez, M., Contreras-Esquivel, J. C., Lara, F., Rodriguez, R., & Aguilar, C. N. (2005). Section C, Journal of Biosciences (Zeitschrift fur Naturforschung), 60, 844–848.

    CAS  Google Scholar 

  14. Mahapatra, S., & Banerjee, D. (2009). Hyalopus sp. Journal of General and Applied Microbiology, 55, 255–259.

    Article  CAS  Google Scholar 

  15. Chhokar, V., Seema, V., & Beniwal, V. (2010). Biotechnology and Bioprocess Engineering, 15, 793–799. doi:10.1007/s12257-010-0058-3.

    Article  CAS  Google Scholar 

  16. Belur, P. D., Gopal, M., Nirmala, K. R., & Basavaraj, N. (2010). Journal of Microbiology and Biotechnology, 20, 732–736.

    CAS  Google Scholar 

  17. Purohit, J. S., Dutta, J. R., Nanda, R. K., & Banerjee, R. (2006). Bioresource Technology, 97, 795–801. doi:10.1016/j.biortech.2005.04.031.

    Article  CAS  Google Scholar 

  18. Raaman, N., Mahendran, B., Jaganathan, C., Sukumar, S., & Chandrasekaran, V. (2010). World Journal of Microbiology and Biotechnology, 26, 1033–1039. doi:10.1007/s11274-009-0266-1.

    Article  CAS  Google Scholar 

  19. Gupta, R., Bradoo, S., & Saxena, R. K. (1997). Letters in Applied Microbiology, 24, 253–255. doi:10.1046/j.1472-765X.1997.00054.x.

    Article  CAS  Google Scholar 

  20. Curiel, J. A., Rodriguez, H., Acebron, I., Mancheno, J. M., de Blanca Rivas, L., & Munoz, R. (2009). Journal of Agricultural and Food Chemistry, 57, 6224–6230. doi:10.1021/jf901045s.

    Article  CAS  Google Scholar 

  21. Murugan, K., Saravanababu, S., & Arunachalam, M. (2007). Bioresource Technology, 98, 946–949. doi:10.1016/j.biortech.2006.04.031.

    Article  CAS  Google Scholar 

  22. Tuite, j. (1969). Plant pathological methods fungi and bacteria (pp.229). U.S.A: Burgess publishing company.

    Google Scholar 

  23. Rout, S., & Banerjee, R. (2006). Indian Journal of Biotechnology, 5, 346–350.

    CAS  Google Scholar 

  24. Ibuchi, S., Minoda, Y., & Yamada, K. (1967). Agricultural and Biological Chemistry, 31, 513–518.

    Article  Google Scholar 

  25. Sharma, S., Agarwal, L., & Saxena, R. K. (2008). Bioresource Technology, 99, 2544–2551. doi:10.1016/j.biortech.2007.04.035.

    Article  CAS  Google Scholar 

  26. Davidson, J., & Schiestl, R. (2000). Cancer Cell Biology. 665 Huntington Avenue, Boston, MA 02115, USA: Harvard School Public Health.

    Google Scholar 

  27. Sharma, S., Bhat, T. K., & Dawra, R. K. (1999). World Journal of Microbiology and Biotechnology, 15, 673–677. doi:10.1023/A:1008939816281.

    Article  CAS  Google Scholar 

  28. Mondal, K. C., Banerjee, D., Banerjee, R., & Pati, B. R. (2001). Journal of General and Applied Microbiology, 47, 263–267. doi:10.2323/jgam.47.263.

    Article  CAS  Google Scholar 

  29. Kasieczka-Burnecka, M., Kuc, K., Kalinowska, H., Knap, M., & Turkiewicz, M. (2007). Applied Microbiology and Biotechnology, 77, 77–89. doi:10.1007/s00253-007-1124-4.

    Article  CAS  Google Scholar 

  30. Ramirez-Coronel, M. A., Viniegra-Gonzalez, G., Darvill, A., & Augur, C. (2003). Microbiology, 149, 2941–2946. doi:10.1099/mic.0.26346-0.

    Article  CAS  Google Scholar 

  31. BeeNa, P. S., Soorej, M. B., Elyas, K. K., Sarita, G. B., & Chandrasekaran, M. (2010). Journal of Microbiology and Biotechnology, 20, 1403–1414. doi:10.4014/jmb.1004.04038.

    Article  CAS  Google Scholar 

  32. Iwamoto, K., Tsuruta, H., Nishitaini, Y., & Osawa, R. (2008). Systematic and Applied Microbiology, 31, 269–277. doi:10.1016/j.syapm.2008.05.004.

    Article  CAS  Google Scholar 

  33. Barthomeuf, C., Regerat, F., & Pourrat, H. (1994). Journal of Fermentation and Bioengineering, 77, 320–323. doi:10.1016/0922-338X(94)90242-9.

    Article  CAS  Google Scholar 

  34. Yamada, H., Adachi, O., Watanabe, M., & Sato, N. (1968). Agricultural and Biological Chemistry, 32, 1070–1078.

    Article  CAS  Google Scholar 

  35. Abdel-Naby, M. A., Sherif, A. A., El-Tanash, A. B., & Mankarios, A. T. (1999). Journal of Applied Microbiology, 87, 108–114. doi:10.1046/j.1365-2672.1999.00799.x.

    Article  CAS  Google Scholar 

  36. Dixon, M., & Webb, E. C. (1966). Enzymes. London: Longman Green & Co.

    Google Scholar 

  37. Zhong, X., Peng, L., Zheng, S., et al. (2004). Protein Expression and Purification, 36, 165–169. doi:10.1016/j.pep.2004.04.016.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Tarbiat Modares University of Iran for financial support.

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Authors and Affiliations

  1. Food Science and Technology Department, Faculty of Agriculture, Tarbiat Modares University, Ale-Ahmad St., Tehran, Iran

    E. Zakipour-Molkabadi, Z. Hamidi-Esfahani, M. A. Sahari & M. H. Azizi

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  1. E. Zakipour-Molkabadi
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  2. Z. Hamidi-Esfahani
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  3. M. A. Sahari
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  4. M. H. Azizi
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Correspondence to Z. Hamidi-Esfahani.

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Zakipour-Molkabadi, E., Hamidi-Esfahani, Z., Sahari, M.A. et al. Improvement of Strain Penicillium sp. EZ-ZH190 for Tannase Production by Induced Mutation. Appl Biochem Biotechnol 171, 1376–1389 (2013). https://doi.org/10.1007/s12010-013-0436-3

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