Abstract
An ultrasensitive fluorimetric biosensor for the detection of chemical warfare agent sulfur mustard (SM) was developed using its monofunctional analogue. SM is a vesicant and a potent chemical threat owing to its direct toxic effects on eyes, lungs, skin and DNA. This work investigates the quenching action of the analyte on chlorophyll fluorescence as elucidated by nuclear magnetic resonance, Fourier transform infrared spectroscopy and mass spectrometry studies suggesting the electrophilic attack of carbonium ion on nitrogens of the porphyrin moiety of chlorophyll. The properties of immobilisation matrix were optimised and scanning electron microscope observations confirmed improvement in pore size of sol–gels by addition of 32 % (v/v) glycerol, a feature enabling enhanced sensitivity towards the analyte. Chlorophyll embedded sol–gel was treated with increasing concentrations of monofunctional SM and the corresponding drop in maximum fluorescence intensity as measured by emission at 673 nm was observed, which varied linearly and had a detection limit of 7.68 × 10−16 M. The biosensor was found to be 6 orders of magnitude more sensitive than the glass microfibre-based disc biosensor previously reported by us.
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Acknowledgments
The authors thank the Defence Research and Development Establishment (DRDE), Gwalior, Ministry of Defence, Government of India for funding the research work. We are also thankful to IIT, Ropar; NIPER, Mohali and CIL, Punjab University, Chandigarh for providing some of the necessary facilities. We thank Dr. Vikrant Naik, postdoctoral fellow, Department of Materials, ETH Zurich for necessary inputs.
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Kaur, S., Singh, M. & Flora, S.J.S. Quenching Action of Monofunctional Sulfur Mustard on Chlorophyll Fluorescence: Towards an Ultrasensitive Biosensor. Appl Biochem Biotechnol 171, 1405–1415 (2013). https://doi.org/10.1007/s12010-013-0429-2
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DOI: https://doi.org/10.1007/s12010-013-0429-2