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Production of Bioethanol from Fermented Sugars of Sugarcane Bagasse Produced by Lignocellulolytic Enzymes of Exiguobacterium sp. VSG-1

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Abstract

Exiguobacterium sp. VSG-1 was isolated from the soil sample and characterized for the production of lignocellulolytic enzymes. Production of these enzymes by the strain VSG-1 was carried out using steam-exploded sugarcane bagasse (SCB) and found to secrete cellulase, pectinase, mannanase, xylanase, and tannase. The growth and enzyme production were found to be optimum at pH 9.0 and 37 °C. Upon steam explosion of SCB, the cellulose increased by 42 %, whereas hemicelluloses and lignin decreased by 40 and 62 %, respectively. Enzymatic hydrolysis of steam-exploded SCB yielded 640 g/l of total sugars. Fermentation of sugars produced from pretreated SCB was carried out by using Saccharomyces cerevisiae at pH 5.0 and 30 °C. The alcohol produced was calculated and found to be 62.24 g/l corresponding to 78 % of the theoretical yield of ethanol. Hence, the strain VSG-1 has an industrial importance for the production of fermentable sugars for biofuels.

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Acknowledgments

This research was supported by research grants to KS from DST and UGC-SAP, the Government of India, New Delhi. VS thank UGC-SAP for providing JRF. We are grateful to Dr. Anu Appaiah for providing facilities to carry out the experiments in CFTRI.

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Authors and Affiliations

  1. Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India

    S. Vijayalaxmi, V. H. Mulimani & K. Sreeramulu

  2. Department of Food Microbiology, Central Food Technological Research Institute, Mysore, 570020, Karnataka, India

    K. A. Anu Appaiah

  3. Agricultural Research Station, University of Agricultural Sciences-Raichur, Gulbarga, 585103, Karnataka, India

    S. K. Jayalakshmi

Authors
  1. S. Vijayalaxmi
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  2. K. A. Anu Appaiah
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  3. S. K. Jayalakshmi
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  4. V. H. Mulimani
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  5. K. Sreeramulu
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Correspondence to K. Sreeramulu.

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Vijayalaxmi, S., Anu Appaiah, K.A., Jayalakshmi, S.K. et al. Production of Bioethanol from Fermented Sugars of Sugarcane Bagasse Produced by Lignocellulolytic Enzymes of Exiguobacterium sp. VSG-1. Appl Biochem Biotechnol 171, 246–260 (2013). https://doi.org/10.1007/s12010-013-0366-0

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