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A Chemiluminescence Microarray Based on Catalysis by CeO2 Nanoparticles and Its Application to Determine the Rate of Removal of Hydrogen Peroxide by Human Erythrocytes

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Abstract

In this work, cerium oxide nanoparticles are capable of strongly enhancing the chemiluminescence (CL) of the luminol–hydrogen peroxide (H2O2) system. Based on this, a microarray CL method for the determination of the removal rate constant of H2O2 by human erythrocytes has been developed. It is providing direct evidence for a H2O2-removing enzyme in human erythrocytes that acts as the predominant catalyst. A reaction mechanism is discussed. The proposed microarray CL method is sensitive, selective, simple and time-saving, and has good reproducibility and high throughput. Relative CL intensity is linearly related to the concentration of H2O2 in the range from 0.01 to 50 μM. The limit of detection is as low as 6.5 × 10−11 M (3σ), and the relative standard deviation is 2. 1 % at 1 μM levels of H2O2 (for n = 11).

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Acknowledgments

This work was supported financially by the Fundamental Research Funds for the Central Universities (program no. GK20091004) and National Natural Science Foundation of China (no. 30872371).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, China

    Xiaohua Li, Zhujun Zhang, Liang Tao, Yongbo Li & Yun yun Li

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  1. Xiaohua Li
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  2. Zhujun Zhang
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  3. Liang Tao
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Correspondence to Zhujun Zhang.

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Li, X., Zhang, Z., Tao, L. et al. A Chemiluminescence Microarray Based on Catalysis by CeO2 Nanoparticles and Its Application to Determine the Rate of Removal of Hydrogen Peroxide by Human Erythrocytes. Appl Biochem Biotechnol 171, 63–71 (2013). https://doi.org/10.1007/s12010-013-0345-5

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  • DOI: https://doi.org/10.1007/s12010-013-0345-5

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