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Effectiveness of Sal Deoiled Seed Cake as an Inducer for Protease Production from Aeromonas sp. S1 for its Application in Kitchen Wastewater Treatment

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Abstract

The present study is an attempt to demonstrate the feasibility of sal (Shorea robusta) deoiled cake—a forest-based industrial by-product—as a cheaper media supplement for augmented protease production from Aeromonas sp. S1 and application of protease in the treatment of kitchen wastewater. Under optimized conditions, protease production could successfully be enhanced to 5.13-fold (527.5 U mL−1) on using sal deoiled seed cake extract (SDOCE), as medium additive, compared to an initial production of 102.7 U mL−1 in its absence. The culture parameters for optimum production of protease were determined to be incubation time (48 h), pH (7.0), SDOCE concentration (3 % (v/v)), inoculum size (0.3–0.6 % (v/v)), and agitation rate (100 rpm). The enzyme was found to have an optimum pH and temperature of 8.0 and 60 °C, respectively. The protease preparation was tested for treatment of organic-laden kitchen wastewater. After 96 h of wastewater treatment under static condition, enzyme preparation was able to reduce 74 % biological oxygen demand, 37 % total suspended solids, and 41 % oil and grease. The higher and improved level of protease obtained using sal deoiled seed cake-based media hence offers a new approach for value addition to this underutilized biomass through industrial enzyme production. The protease produced using this biomass could also be used as pretreatment tool for remediation of organic-rich food wastewater.

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Acknowledgments

The financial support provided by TRIFED (Tribal Co-Operative Marketing and Development Federation of India Limited), Ministry of Tribal Affairs, Government of India is gratefully acknowledged. A.B. is grateful to the University Grants Commission (UGC), Government of India for a Senior Research Fellowship.

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  1. University School of Environment Management, Guru Gobind Singh Indraprastha University, Sector 16-C, Dwarka, New Delhi, 110078, India

    Vandana Saini, Amrik Bhattacharya & Anshu Gupta

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  1. Vandana Saini
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  2. Amrik Bhattacharya
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  3. Anshu Gupta
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Correspondence to Anshu Gupta.

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Saini, V., Bhattacharya, A. & Gupta, A. Effectiveness of Sal Deoiled Seed Cake as an Inducer for Protease Production from Aeromonas sp. S1 for its Application in Kitchen Wastewater Treatment. Appl Biochem Biotechnol 170, 1896–1908 (2013). https://doi.org/10.1007/s12010-013-0323-y

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