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The Solid-State Cultivation of Streptococcus zooepidemicus in Polyurethane Foam as a Strategy for the Production of Hyaluronic Acid

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Abstract

Hyaluronic acid (HA) is a biopolymer with important applications in the pharmaceutical, medical, and cosmetic fields. This work explores the potentialities of a cylindrical polyurethane foam dowel with central aeration as a novel packed bed bioreactor for the production of HA. The goals were to provide a large surface area for oxygen transfer through the patches of liquid film that form in the pores of the foam in which cell proliferation and HA production occur and to easily recover the HA produced. The resulting yields of HA/cell were higher than 1, and the produced HA was completely recovered by pressing the foam. The external conditions that inhibit catabolism, the deviation of energetic metabolism toward the production of HA, were modulated by aeration and the initial glucose concentration. The production of HA was reproducible in 12 successive fermentation cycles. These findings contribute to the development of efficient strategies for the controlled production and recovery of HA.

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Acknowledgments

The authors acknowledge FAPESP for the financial support.

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Authors and Affiliations

  1. School of Chemical Engineering, Department of Materials and Bioprocesses Engineering, University of Campinas, 13083-852, Campinas, SP, Brazil

    Felipe Augusto Ferrari, Fernanda Lopes Motta & Maria Helena Andrade Santana

  2. Center of Agricultural Sciences, Federal University of São Carlos, São Carlos, Brazil

    Reinaldo Gaspar Bastos

Authors
  1. Felipe Augusto Ferrari
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  2. Fernanda Lopes Motta
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  3. Reinaldo Gaspar Bastos
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  4. Maria Helena Andrade Santana
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Correspondence to Maria Helena Andrade Santana.

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Ferrari, F.A., Motta, F.L., Bastos, R.G. et al. The Solid-State Cultivation of Streptococcus zooepidemicus in Polyurethane Foam as a Strategy for the Production of Hyaluronic Acid. Appl Biochem Biotechnol 170, 1491–1502 (2013). https://doi.org/10.1007/s12010-013-0293-0

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  • DOI: https://doi.org/10.1007/s12010-013-0293-0

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