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Molecular Characterization, Expression Patterns, and Association Analysis with Carcass Traits of Porcine USF1 Gene

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Abstract

The upstream stimulatory factor 1 (USF1) is a transcription factor controlling expression of several genes involved in lipid and glucose homeostasis. In this study, two isoforms of the porcine USF1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR), termed USF1 wild-type (wt) and USF1/CD, both of them contain a helix–loop–helix leucine zipper (HLH-LZ) conserved domain. Tissue distribution analysis showed that the two transcripts of porcine USF1 gene were ubiquitously expressed in all tested tissues, except for heart. Moreover, we found that a single nucleotide polymorphism (SNP, C/T) in intron 10 was significantly associated with ratio of lean to fat (P < 0.05), dress percentage (P < 0.05), average backfat thickness (P < 0.05), loin eye width (P < 0.05), lean meat percentage (P < 0.01), loin eye height (P < 0.01), and loin eye area (P < 0.01). This result suggests that porcine USF1 gene may be a candidate gene of meat production trait.

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Acknowledgments

This study was supported financially by National Natural Science Foundation of China (31201791), the Science Foundation for Young Scholars of Hubei Academy of Agricultural Sciences (2012NKYJJ10), Hubei Key Laboratory of Animal Embryo and Molecular Breeding (2012ZD143), (2013ZD102), the Public Welfare Scientific Research Project of Hubei Province (2012DBA21), Hubei Innovation Center of Agricultural Sciences and Technology(2011-620-001-003), National Science and Technology Support Project (2011BAD28B01), National Live Pig Industry Technology System (CARS-36).

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Authors and Affiliations

  1. Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    Huayu Wu, Mu Qiao, Xianwen Peng, Junjing Wu, Guisheng Liu, Hua Sun, Lianghua Li & Shuqi Mei

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  1. Huayu Wu
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  2. Mu Qiao
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  3. Xianwen Peng
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  4. Junjing Wu
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  5. Guisheng Liu
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  6. Hua Sun
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  7. Lianghua Li
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  8. Shuqi Mei
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Correspondence to Shuqi Mei.

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Huayu Wu and Mu Qiao contributed equally to this work.

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Wu, H., Qiao, M., Peng, X. et al. Molecular Characterization, Expression Patterns, and Association Analysis with Carcass Traits of Porcine USF1 Gene. Appl Biochem Biotechnol 170, 1310–1319 (2013). https://doi.org/10.1007/s12010-013-0280-5

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  • DOI: https://doi.org/10.1007/s12010-013-0280-5

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