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Antioxidant, Anti-inflammatory, and Hypoglycemic Effects of the Leaf Extract from Passiflora nitida Kunth

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Abstract

Diabetes mellitus is a metabolic disease characterized by abnormally high plasma glucose levels, leading to major complications, such as insulin resistance, obesity, hyperlipidemia, and hypertension, also with alterations in the immune and neuronal systems. Brazilian plants have been studied as important sources for new molecules with medicinal properties. The genus Passiflora known as “Maracujá” has been used as a traditional folk medicine for a long time, so an investigation was performed regarding an endemic kind of passion fruit (Passiflora nitida Kunth) from Amazonas, Brazil. Here, we aimed to determine its potential biological activity against metabolic syndrome, oxidative stress, pain, and inflammation. The hydroethanol leaf extract revealed an in vitro α-glucosidase inhibitory activity of 50 % inhibitory concentration (IC50) = 6.78 ± 0.31 μg/mL and an α-amylase inhibition of IC50 = 93.36 ± 4.37. In vivo, experiments of different saccharide tolerance resulted in significant glycemia control and, with alloxan-diabetic mice, resulted in a decrease of total cholesterol, a hypoglycemic effect, and an antioxidant activity by thiobarbituric acid-reactive substances measurement. Also, it decreased the carrageenan-induced edema volume and the rate of writhing as a nociceptive response. These results indicate positive effects of P. nitida extract and its potential to inhibit metabolic syndrome.

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Abbreviations

INPA:

National Institute for Amazonian Research or Instituto Nacional de Pesquisas da Amazônia

VRM:

Vegetable raw material

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

BHT:

Butylated hydroxytoluene

NO:

Nitric oxide

NPS:

Nitroprusside solution

PN:

Passiflora nitida

TBARS:

Thiobarbituric acid-reactive substances

w/v:

Weight per volume

IC50 :

50 % inhibitory concentration

TA:

Tannic acid

COX:

Cyclooxygenase

FAPEAM:

Foundation for the Support of Research in the State of Amazonas

CNPq:

Council for Scientific and Technological Development

INCT:

Institutos Nacionais de Ciência e Tecnologia or National Institutes of Science and Technology

DCR:

Programa de Desenvolvimento Científico Regional or Program of Scientific Regional Development

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Acknowledgments

The authors are grateful for the financial support provided by the Foundation for the Support of Research in the State of Amazonas (FAPEAM) and the National Council for Scientific and Technological Development (CNPq). E.S.L. and H.R.M. are members of the INCT de Processos Redox em Biomedicina-Redoxoma (MCT/CNPq). A.P.A.B is a researcher of Programa de Desenvolvimento Científico Regional (DCR-FAPEAM). Jim Hesson of Academic English Solutions.com proofread the text.

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Authors and Affiliations

  1. Laboratory of Biological Activity, Faculty of Pharmaceutical Sciences, Amazonas Federal University, Alexandre Amorim Street, 330, 69010-300, Aparecida, Manaus, Amazonas, Brazil

    Carlos Victor Montefusco-Pereira, Maria José de Carvalho, Ana Paula de Araújo Boleti, Lorisa Simas Teixeira & Emerson Silva Lima

  2. Laboratory of Biochemistry, Department of Physiology, Center of Health and Biological Sciences, Sergipe Federal University, Marechal Rondon Street, Room 14, Jardim Rosa Elze, 49000-100, São Cristovão, Sergipe, Brazil

    Humberto Reis Matos

Authors
  1. Carlos Victor Montefusco-Pereira
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  2. Maria José de Carvalho
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  3. Ana Paula de Araújo Boleti
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  4. Lorisa Simas Teixeira
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  5. Humberto Reis Matos
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  6. Emerson Silva Lima
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Correspondence to Emerson Silva Lima.

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Montefusco-Pereira, C.V., de Carvalho, M.J., de Araújo Boleti, A.P. et al. Antioxidant, Anti-inflammatory, and Hypoglycemic Effects of the Leaf Extract from Passiflora nitida Kunth. Appl Biochem Biotechnol 170, 1367–1378 (2013). https://doi.org/10.1007/s12010-013-0271-6

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  • DOI: https://doi.org/10.1007/s12010-013-0271-6

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