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Optimized Fed-Batch Fermentation of Scheffersomyces stipitis for Efficient Production of Ethanol from Hexoses and Pentoses

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Abstract

Scheffersomyces stipitis was cultivated in an optimized, controlled fed-batch fermentation for production of ethanol from glucose–xylose mixture. Effect of feed medium composition was investigated on sugar utilization and ethanol production. Studying influence of specific cell growth rate on ethanol fermentation performance showed the carbon flow towards ethanol synthesis decreased with increasing cell growth rate. The optimum specific growth rate to achieve efficient ethanol production performance from a glucose-xylose mixture existed at 0.1 h−1. With these optimized feed medium and cell growth rate, a kinetic model has been utilized to avoid overflow metabolism as well as to ensure a balanced feeding of nutrient substrate in fed-batch system. Fed-batch culture with feeding profile designed based on the model resulted in high titer, yield, and productivity of ethanol compared with batch cultures. The maximal ethanol concentration was 40.7 g/L. The yield and productivity of ethanol production in the optimized fed-batch culture was 1.3 and 2 times higher than those in batch culture. Thus, higher efficiency ethanol production was achieved in this study through fed-batch process optimization. This strategy may contribute to an improvement of ethanol fermentation from lignocellulosic biomass by S. stipitis on the industrial scale.

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Acknowledgment

This work has been supported by Thailand Research Fund (TRF) Grant for New Researcher.

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Authors and Affiliations

  1. Biochemical Engineering and Pilot Plant Research and Development Unit, King Mongkut’s University of Technology Thonburi, 49 Soi Tientalay 25, Bangkhuntien-Chaytalay Rd, Thakhum, Bangkhuntien, Bangkok, 10150, Thailand

    Pornkamol Unrean & Nhung H.A. Nguyen

  2. National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand

    Pornkamol Unrean

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  1. Pornkamol Unrean
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  2. Nhung H.A. Nguyen
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Correspondence to Pornkamol Unrean.

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Unrean, P., Nguyen, N.H. Optimized Fed-Batch Fermentation of Scheffersomyces stipitis for Efficient Production of Ethanol from Hexoses and Pentoses. Appl Biochem Biotechnol 169, 1895–1909 (2013). https://doi.org/10.1007/s12010-013-0100-y

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