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Purification and Biochemical Characterization of a Novel Alkaline (Phospho)lipase from a Newly Isolated Fusarium solani Strain

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Abstract

An extracellular lipase from Fusarium solani strain (F. solani lipase (FSL)) was purified to homogeneity by ammonium sulphate precipitation, gel filtration and anion exchange chromatography. The purified enzyme has a molecular mass of 30 kDa as estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The 12 NH2-terminal amino acid residues showed a high degree of homology with a putative lipase from the fungus Necteria heamatoccocae. It is a serine enzyme, like all known lipases from different origins. Interestingly, FSL has not only lipase activity but also a high phospholipase activity which requires the presence of Ca2+ and bile salts. The specific activities of FSL were about 1,610 and 2,414 U/mg on olive oil emulsion and egg-yolk phosphatidylcholine as substrates, respectively, at pH 8.0 and 37 °C. The (phospho)lipase enzyme was stable in the pH range of 5–10 and at temperatures below 45 °C.

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Abbreviations

FSL:

Fusarium solani lipase

NaTDC:

Sodium taurodeoxycholic acid

PC:

Phosphatidylcholine

SHL:

Staphylococcus hyicus lipase

TC4 :

Tributyrin

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Acknowledgements

This work is a part of a doctoral thesis by Raida Jallouli whose research was supported financially by Ministère de l’enseignement supérieur et de la recherche scientifique-Tunisia through a grant to Laboratoire de Biochimie et de Génie Enzymatique des Lipases-ENIS. We thank Prof. Hafedh Mejdoub (FSS) for the sequencing of the NH2-terminale.

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Authors and Affiliations

  1. Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038, Sfax, Tunisia

    Raida Jallouli, Fatma Khrouf, Ahmed Fendri, Tahar Mechichi, Youssef Gargouri & Sofiane Bezzine

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  1. Raida Jallouli
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  2. Fatma Khrouf
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Correspondence to Sofiane Bezzine.

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Jallouli, R., Khrouf, F., Fendri, A. et al. Purification and Biochemical Characterization of a Novel Alkaline (Phospho)lipase from a Newly Isolated Fusarium solani Strain. Appl Biochem Biotechnol 168, 2330–2343 (2012). https://doi.org/10.1007/s12010-012-9940-0

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