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Effects of Ion Beams Pretreatment on Damage of UV-B Radiation on Seedlings of Winter Wheat (Triticum aestivum L.)

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Abstract

The seeds of winter wheat were pretreated with three different doses of low-energy N+ beams, and its seedlings were subjected to UV-B irradiation (10.08 kJ m−2 day−1) at three-leaves stage. The growth characteristic of seeds, the oxidative damage to membrane system induced by UV-B radiation, and the alleviating effects of N+ beams pretreatment to radiation damage were investigated. The results showed that the germination rate and seedling rate, respectively, increased 14.09 ± 1.03 and 13.91 ± 1.21 % compared with control (CK) at the dose of 4.0 × 1016 ions/cm2. When seedlings were exposed to UV-B radiation, the pretreatment method under the dose of 4.0 × 1016 ions/cm2 made the activity of peroxidase and superoxide dismutase increasing, the content of chlorophyll enhancing, but the content of malondialdehyde reducing significantly compared with that of the single UV-B radiation. Whereas, the activity of catalase irradiated by UV-B improved notably under the pretreatment dose of 8.0 × 1016 ions/cm2. In addition, after being irradiated with UV-B, the content of soluble protein and glutathione whose seeds were pretreated by the dose of 6.0 × 1016 ions/cm2 were higher than that of the single UV-B radiation. It was suggested that the suitable dose of low-energy ion beams pretreatment to wheat seeds could change its physiological characteristics at seedlings stage to alleviate the damage effects from UV-B radiation.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (no. 10505018) and the Natural Science Foundation of Henan Province of P. R. of China (no. 091100110401).

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Authors and Affiliations

  1. Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, People’s Republic of China

    Shuaipeng Zhao, Qunce Huang, Pengming Yang, Jiajia Zhang, Hongru Jia & Zhen Jiao

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  1. Shuaipeng Zhao
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  2. Qunce Huang
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  3. Pengming Yang
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Correspondence to Shuaipeng Zhao.

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Zhao, S., Huang, Q., Yang, P. et al. Effects of Ion Beams Pretreatment on Damage of UV-B Radiation on Seedlings of Winter Wheat (Triticum aestivum L.). Appl Biochem Biotechnol 168, 2123–2135 (2012). https://doi.org/10.1007/s12010-012-9922-2

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