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Bioactives of Microbes Isolated from Western Ghat Belt of Kerala Show β-Lactamase Inhibition along with Wide Spectrum Antimicrobial Activity

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Abstract

The present study describes the exploitation of microbial biodiversity from Western Ghats of Kerala for screening of bioactives having β-lactamase inhibitory activities. A total of 700 pure cultures were isolated and were screened for antibacterial activity against a β-lactam resistant Bacillus cereus strain (PL 10) isolated from the same niche. Bioactive extracts made from 45 isolates showed inhibitory activities against PL 10, of which two strains showed inhibition of extended spectrum β-lactamase (ESBL) producing Klebsiella ESBL1101 and three strains inhibited methicillin-resistant Staphylococcus aureus (MRSA) strain MRSA831. All these five strains showed wide spectrum antimicrobial activity against various fungi and bacteria. These five cultures were identified by 16S rRNA sequencing and biochemical tests and the preliminary characterizations of their bioactive extracts were carried out. This study suggests the potential of bioactives from two inhibitor–producer strains, NII 167 and NII 1054, for being developed as inhibitors against wide spectrum β-lactam resistant strains.

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Acknowledgements

The work was funded by CSIR, New Delhi under the Task force programme, NWP 006. The permission from Forest Department, Kerala State, India for allowing soil sample collection from Silent Valley National Park is greatly appreciated.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, 695019, Kerala, India

    Sowmya Pazhur Mohandas, Sita Ravikumar, Sumi J Menachery, Gayathri Suseelan, Sai Shyam Narayanan & Kesavan Madhavan Nampoothiri

  2. Extract Making and Bioactive Screening, Institute of Microbial Technology, Sector-39A, Chandigarh, 160036, India

    Hemaraj Nandanwar

Authors
  1. Sowmya Pazhur Mohandas
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  2. Sita Ravikumar
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  3. Sumi J Menachery
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  4. Gayathri Suseelan
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  5. Sai Shyam Narayanan
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  6. Hemaraj Nandanwar
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  7. Kesavan Madhavan Nampoothiri
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Corresponding author

Correspondence to Kesavan Madhavan Nampoothiri.

Additional information

Sowmya P. Mohandas, Sita Ravikumar, Sumi J Menachery, Gayathri Suseelan contributed equally to the work.

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Mohandas, S.P., Ravikumar, S., Menachery, S.J. et al. Bioactives of Microbes Isolated from Western Ghat Belt of Kerala Show β-Lactamase Inhibition along with Wide Spectrum Antimicrobial Activity. Appl Biochem Biotechnol 167, 1753–1762 (2012). https://doi.org/10.1007/s12010-012-9596-9

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  • DOI: https://doi.org/10.1007/s12010-012-9596-9

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