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Characterization of a broad range antibacterial substance from a new Bacillus species isolated from Amazon basin

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Abstract

A Bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16S rDNA sequencing. The phylogenetic analysis indicated that this strain has low sequence similarity with most Bacillus spp., suggesting a new species was isolated. The antimicrobial activity was detected starting at the exponential growth phase, and maximum activity was observed at stationary phase. The substance was inhibitory to a broad range of indicator strains, incluing pathogenic and food spoilage bacteria such as Listeria monocytogenes, B. cereus, Aeromonas hydrophila, Erwinia carotovora, Pasteurella haemolytica, Salmonella Gallinarum, among other. The antibacterial substance was stable over a wide pH range, but it was sensitive to pronase E and lipase. The antibacterial substance was bactericidal and bacteriolytic to L. monocytogenes and B. cereus at 160 AU ml−1. The identification of a broad range bacteriocin-like inhibitory substance active against L. monocytogenes addresses an important aspect of food protection against pathogens and spoilage microorganisms.

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Acknowledgments

Authors thank Dr. Spartaco Astolfi-Filho from Universidade Federal do Amazonas for kindly provide the bacterial strain P34, Centro de Microscopia Eletronica of UFRGS and ULBRA for technical support in electron microscopy. This work received financial support from CNPq and CAPES, Brazil.

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Authors and Affiliations

  1. Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, Brazil

    Amanda S. Motta & Adriano Brandelli

  2. Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil

    Fabiana S. Cannavan & Siu-Mui Tsai

Authors
  1. Amanda S. Motta
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  2. Fabiana S. Cannavan
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  3. Siu-Mui Tsai
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  4. Adriano Brandelli
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Correspondence to Adriano Brandelli.

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Communicated by Jean-Luc Pernodet.

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Motta, A.S., Cannavan, F.S., Tsai, SM. et al. Characterization of a broad range antibacterial substance from a new Bacillus species isolated from Amazon basin. Arch Microbiol 188, 367–375 (2007). https://doi.org/10.1007/s00203-007-0257-2

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  • DOI: https://doi.org/10.1007/s00203-007-0257-2

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