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Chemical Modification of Ethyl Cellulose-Based Highly Porous Membrane for the Purification of Immunoglobulin G

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Abstract

The chemical modification of developed ethyl cellulose-based membrane was carried out to make it suitable for bioseparation. The different reagents were used for the modification of membrane to couple protein A (PA) to study the purification of immunoglobulin G (IgG) from blood. The chemical modification was carried out using relatively simple and mild reaction conditions. The attenuated total reflectance Fourier transform infrared analysis of chemically modified membrane showed new peak at 1,596.06 and 1,716.49 cm−1. The scanning electron microscopy of PA-coupled membrane, which was used for IgG purification showed open pores and 950 ± 21.5 LMH (L m−2 h−1) operational flux at 0.5-bar out pressure. The flux of unmodified membrane was 1,746 ± 18.5 LMH at 0.5-bar out pressure. The equilibrium adsorption concentration (318.5 ± 5.9 μg cm−2) was obtained at 3 h. The adsorption character of PA-coupled membrane was consistent with the Langmuir adsorption model and the non-specific binding was 67.08 ± 1.3 μg cm−2. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed similar purification pattern for purified IgG from human serum and commercial preparation of IgG. All the results have suggested a high potential of PA-coupled ethyl cellulose-based membrane for large-scale purification of IgG.

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References

  1. Castilho, L. R., Anspach, F. B., & Deckwer, W.-D. (2002). Journal of Membrane Science, 207, 253–264.

    Article  CAS  Google Scholar 

  2. Barroso, T., Temtem, M., Hussain, A., Aguiar-Ricardo, A., & Roque, A. C. A. (2010). Journal of Membrane Science, 348, 224–230.

    Article  CAS  Google Scholar 

  3. Bresolin, I. T. L., Fioritti, R. R., & Bueno, S. M. A. (2011). Process Biochemistry, 46, 2277–2285.

    Article  CAS  Google Scholar 

  4. Wongchuphan, R., Tey, B. T., Tan, W. S., Subramanian, S. K., Taip, F. S., & Ling, T. C. (2011). Process Biochemistry, 46, 101–107.

    Article  CAS  Google Scholar 

  5. Suck, K., Walter, J., Menzel, F., Tappe, A., Kasper, C., Naumannb, C., Zeidler, R., & Scheper, T. (2006). Journal of Biotechnology, 121, 361–367.

    Article  CAS  Google Scholar 

  6. Liu, S., Zeng, J., Tao, D., & Zhang, L. (2010). Cellulose, 17, 1159–1169.

    Article  CAS  Google Scholar 

  7. Gemeiner, P., Polakovič, M., Mislovičová, D., & Štefuca, V. J. (1998). Chromatography B, 715, 245–271.

    Article  CAS  Google Scholar 

  8. Boeden, H.-F., Pommerening, K., Becker, M., Rupprich, C., Holtzhauer, M., Loth, F., & Müller, R. (1991). Journal of Chromatography. A, 552, 389–414.

    Article  CAS  Google Scholar 

  9. Hari, P. R., Paul, W., & Sharma, C. P. (2000). Journal of Biomedical Materials Research, 50, 110–113.

    Article  CAS  Google Scholar 

  10. Chandel, A. K., Rao, L. V., Narasu, M. L., & Singh, O. V. (2008). Enzyme and Microbial Technology, 42, 199–207.

    Article  CAS  Google Scholar 

  11. Malakian, A., Golebiowska, M., & Bellefeuille, J. (1993). American Laboratory, 25, 40P–40V.

    CAS  Google Scholar 

  12. Hou, K. C., Zaniewski, R., & Roy, S. (1991). Biotechnology and Applied Biochemistry, 13, 257–268.

    CAS  Google Scholar 

  13. Jia, L., Yang, L., Zou, H., Zhang, Y., Zhao, J., Fan, C., & Sha, L. (1999). Biomedical Chromatography, 13, 472–477.

    Article  CAS  Google Scholar 

  14. Yang, L., & Chen, P. (2002). Journal of Membrane Science, 205, 141–153.

    Article  CAS  Google Scholar 

  15. Charcosset, C. (1998). Journal of Chemical Technology and Biotechnology, 71, 95–110.

    Article  CAS  Google Scholar 

  16. Castilho, L. R., Deckwer, W.-D., & Anspach, F. B. (2000). Journal of Membrane Science, 172, 269–277.

    Article  CAS  Google Scholar 

  17. Adikane, H. V., & Thakar, D. M. (2010). Applied Biochemistry and Biotechnology, 160, 1130–1145.

    Article  CAS  Google Scholar 

  18. Wei, X., Yunjun, L., Xuejing, S., Jingru, L., Yin, Y., Yong, L., & Yongbin, J. (2008). Journal of Membrane Science, 325, 592–598.

    Article  Google Scholar 

  19. Shi, P., Zuo, Y., Zou, Q., Shen, J., Zhang, L., Li, Y., & Morsi, Y. S. (2009). International Journal of Pharmaceutics, 375, 67–74.

    Article  CAS  Google Scholar 

  20. Larsson, M., Hjärtstam, J., Berndtsson, J., Stading, M., & Larsson, A. (2010). European Journal of Pharmaceutics and Biopharmaceutics, 76, 428–432.

    Article  CAS  Google Scholar 

  21. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  22. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  23. Ho, Y.-S., Chiu, W.-T., & Wang, C.-C. (2005). Bioresource Technology, 96, 1285–1291.

    Article  CAS  Google Scholar 

  24. Porath, J., & Axén, R. (1976). In K. Mosbach (Ed.), Methods in enzymology, vol, 44: immobilized enzymes (pp. 19–45). New York: Academic.

    Chapter  Google Scholar 

  25. Fang, F., Yu, L., Binyuan, Z., & Ke'ao, H. (2005). J Wuhan Univ Technol Mater Sci, 20, 20–23.

    Article  CAS  Google Scholar 

  26. Park, D., Haam, S., Jang, K., Ahn, I., & Kim, W. (2005). Process Biochemistry, 40, 53–61.

    Article  CAS  Google Scholar 

  27. Beeskow, T. C., Kroner, K. H., & Anspach, F. B. (1997). Journal of Colloid and Interface Science, 196, 278–291.

    Article  CAS  Google Scholar 

  28. Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., & Behr, J.-P. (1995). Proceedings of the National Academy of Sciences of the United States of America, 92, 7297–7301.

    Article  CAS  Google Scholar 

  29. Ukeda, H., Ishii, T., Sawamura, M., & Kusunose, H. (1995). Analytica Chimica Acta, 308, 261–268.

    Article  CAS  Google Scholar 

  30. Zeng, X., & Ruckenstein, E. (1996). Journal of Membrane Science, 117, 271–278.

    Article  CAS  Google Scholar 

  31. Sugiura, T., Imagawa, H., & Kondo, T. (2000). Journal of Chromatography B, 742, 327–334.

    Article  CAS  Google Scholar 

  32. Randy, G. L., & Sung, W. K. (1974). Journal of Biomedical Materials Research, 8, 251–259.

    Article  Google Scholar 

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Acknowledgment

We thank DBT, New Delhi, India, for giving the financial support to carry out the work (BT/PR2937/PID/06/147/2002).

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  1. Chemical Engineering Division, National Chemical Laboratory, Pune, 411 008, India

    H. V. Adikane & G. J. Iyer

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  1. H. V. Adikane
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  2. G. J. Iyer
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Correspondence to H. V. Adikane.

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Adikane, H.V., Iyer, G.J. Chemical Modification of Ethyl Cellulose-Based Highly Porous Membrane for the Purification of Immunoglobulin G. Appl Biochem Biotechnol 169, 1026–1038 (2013). https://doi.org/10.1007/s12010-012-0085-y

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  • DOI: https://doi.org/10.1007/s12010-012-0085-y

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