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Analysis of Nitrification in Agricultural Soil and Improvement of Nitrogen Circulation with Autotrophic Ammonia-Oxidizing Bacteria

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Abstract

Accumulations of inorganic nitrogen (NH4 +, NO2 −, and NO3 −) were analyzed to evaluate the nitrogen circulation activity in 76 agricultural soils. Accumulation of NH4 + was observed, and the reaction of NH4 + → NO2 − appeared to be slower than that of NO2 − → NO3 − in agricultural soil. Two autotrophic and five heterotrophic ammonia-oxidizing bacteria (AOB) were isolated and identified from the soils, and the ammonia-oxidizing activities of the autotrophic AOB were 1.0 × 103–1.0 × 106 times higher than those of heterotrophic AOB. The relationship between AOB number, soil bacterial number, and ammonia-oxidizing activity was investigated with 30 agricultural soils. The ratio of autotrophic AOB number was 0.00032–0.26 % of the total soil bacterial number. The soil samples rich in autotrophic AOB (>1.0 × 104 cells/g soil) had a high nitrogen circulation activity, and additionally, the nitrogen circulation in the agricultural soil was improved by controlling the autotrophic AOBs.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University, 1-1-1, Nojihigashi, Kusatsu, Shiga, 5258577, Japan

    Toshihide Matsuno, Sachie Horii, Takanobu Sato, Yoshiki Matsumiya & Motoki Kubo

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  1. Toshihide Matsuno
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  2. Sachie Horii
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  3. Takanobu Sato
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  4. Yoshiki Matsumiya
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  5. Motoki Kubo
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Correspondence to Motoki Kubo.

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Matsuno, T., Horii, S., Sato, T. et al. Analysis of Nitrification in Agricultural Soil and Improvement of Nitrogen Circulation with Autotrophic Ammonia-Oxidizing Bacteria. Appl Biochem Biotechnol 169, 795–809 (2013). https://doi.org/10.1007/s12010-012-0029-6

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  • DOI: https://doi.org/10.1007/s12010-012-0029-6

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