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Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications

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Abstract

In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.

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Acknowledgments

This research was supported by an EPSRC research fellowship (grant EP/C535456/1, for E. H. Yu). This research was also supported by the US Office of Naval Research (ONR) (N00014-03-1-0026, for R. Prodanovic, R. Ostafe, and U. Schwaneberg).

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Authors and Affiliations

  1. School of Chemical Engineering and Advanced Materials, Merz Court, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK

    Eileen Hao Yu

  2. School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany

    Radivoje Prodanovic, Güray Güven, R. Ostafe & U. Schwaneberg

  3. Institut für Molekulare Biotechnologie, RWTH Aachen, Forckenbeckstrasse 6, 52074, Aachen, Germany

    Radivoje Prodanovic & R. Ostafe

  4. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia

    Radivoje Prodanovic

  5. Lehrstuhl für Biotechnologie, RWTH Aachen, Worringerweg 1, 52074, Aachen, Germany

    U. Schwaneberg

Authors
  1. Eileen Hao Yu
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  2. Radivoje Prodanovic
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  3. Güray Güven
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  4. R. Ostafe
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  5. U. Schwaneberg
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Correspondence to Eileen Hao Yu.

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Yu, E.H., Prodanovic, R., Güven, G. et al. Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. Appl Biochem Biotechnol 165, 1448–1457 (2011). https://doi.org/10.1007/s12010-011-9366-0

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  • DOI: https://doi.org/10.1007/s12010-011-9366-0

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