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Concentration, Partial Characterization, and Immobilization of Lipase Extract from P. brevicompactum by Solid-State Fermentation of Babassu Cake and Castor Bean Cake

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Abstract

One relevant limitation hindering the industrial application of microbial lipases has been attributed to their production cost, which is determined by the production yield, enzyme stability among other. The objective of this work was to evaluate the concentration and immobilization of lipase extracts from Penicillium brevicompactum obtained by solid-state fermentation of babassu cake and castor bean cake. The precipitation with ammonium sulfate 60% of saturation of crude extract obtained with babassu cake as raw material showed an enhancement in hydrolytic and esterification activities from 31.82 to 227.57 U/g and from 170.92 to 207.40 U/g, respectively. Concentrated lipase extracts showed preference to medium-chain triglycerides and fatty acids. It is shown that the enzyme activity is maintained during storage at low temperatures (4 and −10°C) for up to 30 days. Higher esterification activities were achieved when the lipase extract was immobilized in sodium alginate and activated coal.

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Acknowledgements

The authors thank PETROBRAS for the financial support of this work and scholarship.

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Authors and Affiliations

  1. Departamento de Engenharia de Alimentos, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, Brazil

    Marceli Fernandes Silva, Marco Di Luccio, Marcio A. Mazutti, J. Vladimir Oliveira, Helen Treichel & Débora de Oliveira

  2. Departamento de Bioquímica, UFRJ, Instituto de Química, Av. Athos da Silveira Ramos 149, Rio de Janeiro, 21941-909, Brazil

    Denise M. G. Freire

  3. PETROBRAS-CENPES, Centro de Pesquisas e Desenvolvimento Leopoldo Américo Miguez de Melo, Avenida Horácio Macedo 950-Ilha do Fundão, Rio de Janeiro, 21941-915, Brazil

    Aline Machado de Castro

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  1. Marceli Fernandes Silva
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  2. Denise M. G. Freire
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  3. Aline Machado de Castro
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  4. Marco Di Luccio
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  5. Marcio A. Mazutti
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  6. J. Vladimir Oliveira
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  7. Helen Treichel
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  8. Débora de Oliveira
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Correspondence to Helen Treichel.

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Silva, M.F., Freire, D.M.G., de Castro, A.M. et al. Concentration, Partial Characterization, and Immobilization of Lipase Extract from P. brevicompactum by Solid-State Fermentation of Babassu Cake and Castor Bean Cake. Appl Biochem Biotechnol 164, 755–766 (2011). https://doi.org/10.1007/s12010-011-9171-9

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  • DOI: https://doi.org/10.1007/s12010-011-9171-9

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