Abstract
The release of mine effluents can have a damaging impact on receiving water bodies. Therefore, treatment of mine waters before discharge is imperative. A novel biological \( {\hbox{SO}}_4^{2 - } \) removal technology has been developed whereby the degradation/fermentation products of grass cellulose, volatile fatty acids (VFA), function as the electron donors and \( {\hbox{SO}}_4^{2 - } \) as the electron acceptor. The aim of the study presented here was to elucidate the interactions between the cellulose degradation rate, the chemical oxygen demand (COD), VFA production and its/utilisation rate as well as the sulphate reduction rate. To this end, two stirred batch reactors were operated: a test and a control reactor. The results showed that high COD and VFA concentrations were achieved after cellulose degradation, which resulted in a rapid decrease in the \( {\hbox{SO}}_4^{2 - } \) concentration in the test reactor. The VFA results indicated that propionic and butyric acids were preferentially utilised, producing acetate. In the control reactor, the VFA and the COD production increased initially at the same rate, followed later by a decrease at a similar rate. These results suggest that the degradation products formed were utilised by the methanogenic bacteria to produce methane rather than by the sulphate-reducing bacteria, since the control reactor contained no sulphate (Visser 1995). Furthermore, these results showed a clear relationship between the COD/VFA production and the \( {\hbox{SO}}_4^{2 - } \) reduction in the test reactor and between the COD and VFA pattern in the control reactor.
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Younger, P. L., & Wolkersdorfer, C. (2004). Mining impact on the fresh water environment: Technical and managerial guidelines for catchment scale management. Mine Water and the Environment, 23, S2–S80.
Maree, J. P. (1994). Neutralization of acidic effluents with limestone. Water Research Commission Report No. 355/1/94.
Maree, J. P., Hagger, M. J., Strobos, G., Hlabela, P., Cronjé, H., van Niekerk, A., et al. (2003). Neutralization of acid leachate at a nickel mine with limestone. In Proc. Sudbury 2003 Mining and the Environment, 28th CLRA Meeting, Laurentian University, Sudbury, 25–28 May 2003.
Günther, P. (2006). Emalahleni mine water reclamation project. In Proceedings of Mine Water Drainage (South African Perspective). Randfontein, 19–20 Oct 2006.
Hulshoff-Pol, L. W., Lens, P. N. L., Stams, A. J. M., & Lettinga, G. (1998). Anaerobic treatment of sulphate rich wastewaters. Biodegradation, 9, 213–224.
Lens, P. N. L., Visser, A., Janssen, A. J. H., Hulshoff Pol, L. W., & Lettinga, G. (1998). Biotechnological treatment of sulphate-rich wastewaters. Critical Reviews in Environmental Science and Technology, 28(1), 41–88.
De Smul, A., Dries, J., Goethals, L., Grootaerd, H., & Verstraete, W. (1997). High rate of microbial sulphate reduction in a mesophile ethanol fed expanded-granular-sludge-blanket reactor. Applied Microbiology and Biotechnology, 48, 297–303.
Greben, H. A., Maree, J. P., Singmin, Y., & Mnqanqeni, S. (2000). Biological sulphate removal from acid mine effluent using ethanol as carbon and energy source. Water Science and Technology, 42(3–4), 339–344.
Greben, H. A., Baloyi, L. M., Sigama, S., & Venter, S. N. (2009). Bioremediation of sulphate rich mine effluents using grass cuttings and rumen fluid microorganisms. Journal of Geochemical Exploration, 100(2–3), 163–168.
Visser, A. (1995). The anaerobic treatment of sulphate containing wastewater. PhD Thesis, Wageningen Agricultural University, Wageningen, The Netherlands.
Oude Elferink, S. J. W. H. (1998). Sulphate-reducing Bacteria in Anaerobic Bioreactors. PhD Thesis, Wageningen Agricultural University, Wageningen, The Netherlands
Mizuno, O., Li, Y. Y., & Noike, T. (1998). The behaviour of sulphate-reducing bacteria I acidogenic phase of anerobic digestion. Water Research, 32, 1626–1634.
Greben, H. A., Baloyi, L. M., & Venter, S. N. (2007). Grass cellulose as cost effective energy source for biological sulphate removal. Water SA, 33(5), 729–735.
Greben, H. A., Sigama, J., Burke, L., & Venter, S. N. (2008). Cellulose fermentation products as energy sources for biological sulphate reduction. WRC report K5/1728.
Postgate, J. R. (1984). The sulphate-reducing bacteria (2nd ed., p. 208). Cambridge: Cambridge University Press.
Hungate, R. E. (1966). The rumen and its microbes. New York: Academic Press.
APHA. (1985). Standard methods for the examination of water and wastewater (16th ed.). Washington DC: American Public Health Association.
Harmsen, H. J. M. (1996). Detection, phylogeny and population dynamic of synthrophic propionate-oxidizing bacteria in anaerobic sludge. PhD thesis, Wageningen Agricultural University, Wageningen.
Logan, M. V., Reardon, K. F., Figueroa, L. A., Mclain, J. E. T., & Ahmann. (2005). Microbial community activities during establishment, performance and decline of bench-scale passive treatment systems for mine drainage. Water Research, 39, 4537–4551.
Rinzema, A., & Lettinga, G. (1988). Anaerobic treatment of sulfate containing wastewater. In D. L. Wise (Ed.), Biotreatment systems, 3 (pp. 65–109). Boca Raton: CRC Press.
Marschner, B., & Kalbitz, K. (2003). Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma, 113, 211–235.
Lynd, L. R., Weimer, P. J., van Zyl, W., & Pretorius, I. S. (2002). Microbial cellulose utilisation: fundamentals and biotechnology. Microbiology and Molecular Biology Reviews, 66(3), 506–577.
Greben, H. A., & Baloyi, J. (2004a). The beneficial use of a bio-waste product in the biological sulphate removal technology. In Proceedings Wisa Biennial Conference and Exhibition, Cape Town, South Africa, 2–6 May 2004.
Vallero, M. V. G., Lens, P. N. L., Bakker, C., & Lettinga, G. (2003). Sulfidogenic volatile fatty acid degradation in a baffled reactor. Sub-department of Environmental Technology, Wageningen University and Research Centre, Wageningen, The Netherlands.
Greben, H. A., Tjatji, M., & Maree, J. P. (2004b). Biological sulphate reduction at different feed COD/SO4 ratios using propionate and acetate as the energy source. In 8th IMWA Conference Newcastle upon Thyne, England.
Reis, M. A. M., Almeida, J. S., Lemos, P. C., & Carrondo, M. J. T. (1992). Effect of hydrogen sulphide on growth of sulphate-reducing bacteria. Biotechnology and Bioengineering, 40, 593–600.
Sonakya, V., Raizada, N., Dalhoff, R., & Wilderer, P. A. (2003). Water Science and Technology, 48(8), 255–259.
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Mulopo, J., Greben, H., Sigama, J. et al. The Relationships Between Sulphate Reduction and COD/VFA Utilisation Using Grass Cellulose as Carbon and Energy Sources. Appl Biochem Biotechnol 163, 393–403 (2011). https://doi.org/10.1007/s12010-010-9047-4
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DOI: https://doi.org/10.1007/s12010-010-9047-4