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Functional Expression of phaCAB Genes from Cupriavidus taiwanensis Strain 184 in Escherichia coli for Polyhydroxybutyrate Production

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Abstract

Polyhydroxyalkanoates are polyesters synthesized by numerous microorganisms. These polyesters are biodegradable and have similar properties to those of conventional plastics. Cupriavidus taiwanensis strain 184 is phylogenetically related to the well-known polyhydroxybutyrate (PHB) producer Ralstonia eutropha (Cupriavidus necator) and is also shown to be able to accumulate significant amounts of PHB. In this study, we cloned the PHB synthesis genes (phaCAB) from C. taiwanensis 184 into Escherichia coli for biosynthesis of PHB. The recombinant E. coli strains were able to synthesize significant amounts of PHB. The PHB amounted to about 66∼70% of total cell material of these recombinant strains.

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Acknowledgment

We thank Dr. Thomas J Lie, University of Washington, Seattle, WA, USA, for his critical review and editing assistance of this manuscript. The present study was supported partly by grants (contract number NSC98-2622-E-155-003-CC2 and NSC97-2221-E-155-006-MY3) from National Science Council.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Rd, Chung-Li, 320, Taiwan

    Chih-Ching Chien, Chian-Chiao Hong, Yu-Hong Wei & Shan-Yu Chen

  2. Department of Chemical Engineering and Material Sciences, Yuan Ze University, Chung-Li, 320, Taiwan

    Mei-Ling Cheng & Yi-Ming Sun

  3. Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, 970, Taiwan

    Po-Chi Soo

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  1. Chih-Ching Chien
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  2. Chian-Chiao Hong
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  4. Yu-Hong Wei
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Correspondence to Chih-Ching Chien.

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Chien, CC., Hong, CC., Soo, PC. et al. Functional Expression of phaCAB Genes from Cupriavidus taiwanensis Strain 184 in Escherichia coli for Polyhydroxybutyrate Production. Appl Biochem Biotechnol 162, 2355–2364 (2010). https://doi.org/10.1007/s12010-010-9008-y

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  • DOI: https://doi.org/10.1007/s12010-010-9008-y

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