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Production of poly-β-hydroxybutyrate by fed-batch culture of recombinantEscherichia coli

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Summary

A recombinantEscherichia coli strain harboring the PHB biosynthesis genes fromAlcaligenes eutrophus was used to produce poly-β-hydroxybutyrate (PHB) by pH-stat fedbatch culture. Initial glucose concentration for optimal growth was found to be 20g/L from a series of flask cultures. A final PHB concentration of 88.8 g/L could be obtained after 42 hrs of cultivation.

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Authors and Affiliations

  1. BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 305-701, Taejon, Korea

    Beom Soo Kim, Sang Yup Lee & Ho Nam Chang

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  1. Beom Soo Kim
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  2. Sang Yup Lee
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  3. Ho Nam Chang
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Kim, B.S., Lee, S.Y. & Chang, H.N. Production of poly-β-hydroxybutyrate by fed-batch culture of recombinantEscherichia coli . Biotechnol Lett 14, 811–816 (1992). https://doi.org/10.1007/BF01029144

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