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Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin

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Abstract

Supermacroporous poly{2-hydroxyethyl methacrylate-co-[N,N-bis(2,6-diisopropylphenyl)-perylene-3,4,9,10-tetracarboxylic diimide]} [poly(HEMA-co-DIPPER)] monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with DIPPER as functional comonomer and N,N′-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10–50 μm size. Poly(HEMA-co-DIPPER) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(HEMA-co-DIPPER) cryogel was 14.7 g H2O/g dry cryogel. Poly(HEMA-co-DIPPER) cryogel was used in the adsorption/desorption of albumin from aqueous solutions. The nonspecific adsorption of albumin onto plain poly(HEMA) cryogel was very low (3.36 g/g polymer). The maximum amount of albumin adsorption from aqueous solution in acetate buffer was 40.9 mg/g polymer at pH 5.0. It was observed that albumin could be repeatedly adsorbed and desorbed with the poly(HEMA-co-DIPPER) cryogel without significant loss of adsorption capacity.

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Authors and Affiliations

  1. Ege Vocational and Training School, Ege University, Izmir, Turkey

    Nesibe Avcibaşi

  2. Department of Arts Chemistry, Faculty of Science, Adnan Menderes University, Aydin, Turkey

    Murat Uygun & M. Emin Çorman

  3. Department of Biochemistry, Faculty of Science, Ege University, Izmir, Turkey

    Sinan Akgöl

  4. Department of Chemistry, Faculty of Science, Hacettepe University, Ankara, Turkey

    Adil Denizli

Authors
  1. Nesibe Avcibaşi
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  2. Murat Uygun
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  3. M. Emin Çorman
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  4. Sinan Akgöl
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  5. Adil Denizli
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Correspondence to Sinan Akgöl.

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Avcibaşi, N., Uygun, M., Çorman, M.E. et al. Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin. Appl Biochem Biotechnol 162, 2232–2243 (2010). https://doi.org/10.1007/s12010-010-8997-x

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  • DOI: https://doi.org/10.1007/s12010-010-8997-x

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