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Cultivation of Rhodobacter sphaeroides in the Stirred Bioreactor with Different Feeding Strategies for CoQ10 Production

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Abstract

The logistic growth model combined with the Luedeking-Piret equation was adopted in this study to model the batch production of CoQ10 in the cultivation of Rhodobacter sphaeroides. The simulation results indicated that CoQ10 production was a primary metabolite. As being a primary metabolite, a longer cell growing stage would tend to accumulate more biomass and lead to a higher CoQ10 concentration being produced. In this context, a fed-batch operation by molasses feeding was performed to increase the biomass and subsequent CoQ10 production. Three different molasses feeding strategies were operated in this study. Results suggested that the fed-batch operation with molasses controlled at 10 ± 1 g/l could increase the cell mass and CoQ10 concentration to reach their maximum values of 18.6 g/l and 83.8 mg/l, respectively, nearly 2.2 times and 1.9 times their respective values obtained in the batch cultivation.

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References

  1. Kawamukai, M. (2002). Journal of Bioscience & Bioengineering, 94, 51–57.

    Google Scholar 

  2. Lieberman, A., Lyons, K., Levine, J., & Myerburg, R. (2005). Parkinsonism & Related Disorders, 11, 81–84. doi:10.1016/j.parkreldis.2004.07.012.

    Article  Google Scholar 

  3. Choi, J.H., Ryu, Y.W., & Seo, J.H. (2005). Applied Microbiology & Biotechnology, 68, 9–15. doi:10.1007/s00253-005-1946-x.

    Article  CAS  Google Scholar 

  4. Cluis, C.P., Burja, A.M., & Martin, V.J.J. (2007). Trends in Biotechnology, 25, 514–521. doi:10.1016/j.tibtech.2007.08.008.

    Article  CAS  Google Scholar 

  5. Sasaki, K., Watanabe, M., Suda, Y., Ishizuka, A., & Noparatnaraporn, N. (2005). Journal of Bioscience & Bioengineering, 100, 481–488.l. doi:10.1263/jbb.100.481.

    Article  CAS  Google Scholar 

  6. Yoshisa, H., Kotani, Y., Ochiai, K., & Araki, K. (1998). The Journal of General & Applied Microbiology, 44, 19–26. doi:10.2323/jgam.44.19.

    Article  Google Scholar 

  7. Benkortbi, O., Hanini, S., & Bentahar, F. (2007). Biochemical Engineering Journal, 36, 14–18. doi:10.1016/j.bej.2006.06.015.

    Article  CAS  Google Scholar 

  8. Songa, H., Jang, S.H., Park, J.M., & Lee, S.Y. (2007). Biochemical Engineering Journal, 40, 107–115. doi:10.1016/j.bej.2007.11.021.

    Article  Google Scholar 

  9. Ha, S.J., Kim, S.Y., Seo, J.H., Oh, D.K., & Lee, J.K. (2007). Applied Microbiology & Biotechnology, 74, 974–980. doi:10.1007/s00253-006-0744-4.

    Article  CAS  Google Scholar 

  10. Park, Y.C., Kim, S.J., Choi, J.H., Lee, W.H., Park, K.M., Kawamukai, M., et al. (2005). Applied Microbiology & Biotechnology, 67, 192–196. doi:10.1007/s00253-004-1743-y.

    Article  CAS  Google Scholar 

  11. Yen, H.W., & Chiu, C.H. (2007). Enzyme & Microbial Technology, 4, 1600–1604.

    Google Scholar 

  12. Wee, Y.J., Kima, J.N., Yunb, J.S., & Ryua, H.W. (2004). Enzyme & Microbial Technology, 34, 568–573. doi:10.1016/j.enzmictec.2004.08.008.

    Article  Google Scholar 

  13. Luedeking, R., & Piret, E.L. (1959). Journal of Biochemical & Microbiological Technology & Engineering, 1, 393–412. doi:10.1002/jbmte.390010406.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank the National Science Council of the R.O.C. for the financial supports (NSC 95-2221-E-029-007) in this study.

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  1. Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan, Republic of China

    Hong-Wei Yen, Chang-Yu Feng & Jia-Lin Kang

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  1. Hong-Wei Yen
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  2. Chang-Yu Feng
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Correspondence to Hong-Wei Yen.

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Yen, HW., Feng, CY. & Kang, JL. Cultivation of Rhodobacter sphaeroides in the Stirred Bioreactor with Different Feeding Strategies for CoQ10 Production. Appl Biochem Biotechnol 160, 1441–1449 (2010). https://doi.org/10.1007/s12010-009-8576-1

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  • DOI: https://doi.org/10.1007/s12010-009-8576-1

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