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Improving Enzymatic Production of Ginsenoside Rh2 from Rg3 by using Nonionic Surfactant

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Abstract

In this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside Rg3 into Rh2 which was catalyzed at 50 °C and pH 5.0 by a crude glucosidase extracted from Fusarium sp. ECU2042. Among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. After optimizing some influencing factors on the reaction, the conversion of Rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the nonionic surfactant (7.5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (Grant Nos. 20576037 and 20672037), the Ministry of Education (Grant Nos. 20050251011 and B07023), and the Ministry of Science and Technology, P. R. China (Grant Nos. 2006AA02Z205 and 2007AA02Z225).

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Authors and Affiliations

  1. Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Jin-Huan Su & Jian-He Xu

  2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Zhi-Long Wang

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  1. Jin-Huan Su
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  2. Jian-He Xu
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  3. Zhi-Long Wang
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Correspondence to Jian-He Xu.

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Su, JH., Xu, JH. & Wang, ZL. Improving Enzymatic Production of Ginsenoside Rh2 from Rg3 by using Nonionic Surfactant. Appl Biochem Biotechnol 160, 1116–1123 (2010). https://doi.org/10.1007/s12010-009-8570-7

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  • DOI: https://doi.org/10.1007/s12010-009-8570-7

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