Abstract
A central composite design of the response surface methodology (RSM) was employed to study the effects of temperature, enzyme concentration, and stirring rate on recycled-paper enzymatic hydrolysis. Among the three variables, temperature and enzyme concentration significantly affected the conversion efficiency of substrate, whereas stirring rate was not effective. A quadratic polynomial equation was obtained for enzymatic hydrolysis by multiple regression analysis using RSM. The results of validation experiments were coincident with the predicted model. The optimum conditions for enzymatic hydrolysis were temperature, enzyme concentration, and stirring rate of 43.1 °C, 20 FPU g−1 substrate, and 145 rpm, respectively. In the subsequent simultaneous saccharification and fermentation (SSF) experiment under the optimum conditions, the highest 28.7 g ethanol l−1 was reached in the fed-batch SSF when 5% (w/v) substrate concentration was used initially, and another 5% added after 12 h fermentation. This ethanol output corresponded to 77.7% of the theoretical yield based on the glucose content in the raw material.
Similar content being viewed by others
References
Van Wyk, J. P. H., & Mohulatsi, M. (2003). Biodegradation of wastepaper by cellulase from Trichoderma viride. Bioresource Technology, 86, 21–23. doi:10.1016/S0960-8524(02)00130-X.
Duff, S. J. B., & Murray, W. D. (1996). Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Bioresource Technology, 55, 1–33. doi:10.1016/0960-8524(95)00122-0.
Wyman, C. E. (1999). Biomass ethanol: technical progress, opportunities, and commercial challenges. Annual Review of Energy and the Environment, 24, 189–226. doi:10.1146/annurev.energy.24.1.189.
Pan, X. J., & Arato, C. (2005). Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnology and Bioengineering, 90, 473–481. doi:10.1002/bit.20453.
Chen, M., Zhao, J., & Xia, L. M. (2008). Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars. Carbohydrate Polymers, 71, 411–415. doi:10.1016/j.carbpol.2007.06.011.
Wang, G., Mu, Y., & Yu, H. Q. (2005). Response surface analysis to evaluate the influence of pH, temperature and substrate concentration on the acidogenesis of sucrose-rich wastewater. Biochemical Engineering Journal, 23, 175–184. doi:10.1016/j.bej.2005.01.002.
Lu, X. B., Zhang, Y. M., Yang, J., & Liang, Y. (2007). Enzymatic hydrolysis of corn stover after pretreatment with dilute sulfuric acid. Chemical Engineering & Technology, 30, 938–944. doi:10.1002/ceat.200700035.
Khuri, A. I., & Cornell, J. A. (1987). Response surfaces: design and analysis. New York: Marcel Dekker.
Tang, X., He, G. Q., Chen, Q. H., Zhang, X. Y., & Ali, M. A. M. (2004). Medium optimization for the production of thermal stable β-glucanase by Bacillus subtilis ZJF-1A5 using response surface methodology. Bioresource Technology, 93, 175–181. doi:10.1016/j.biortech.2003.10.013.
Shi, S. L. (2003). Analysis and detection of pulping and papermaking (1st ed.). Beijing: Chinese Light Industry Press.
Ghose, T. K. (1987). Measurement of cellulase activities. Pure and Applied Chemistry, 59, 257–268. doi:10.1351/pac198759020257.
Kunamneni, A., & Singh, S. (2005). Response surface optimization of enzymatic hydrolysis of maize starch for higher glucose production. Biochemical Engineering Journal, 27, 179–190. doi:10.1016/j.bej.2005.08.027.
Kaushik, R., Saran, S., Isar, J., & Saxena, R. K. (2006). Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus. Journal of Molecular Catalysis. B, Enzymatic, 40, 121–126. doi:10.1016/j.molcatb.2006.02.019.
Shieh, C. J., Liao, H. F., & Lee, C. C. (2003). Optimization of lipase-catalyzed biodiesel by response surface methodology. Bioresource Technology, 88, 103–106. doi:10.1016/S0960-8524(02)00292-4.
Cui, F. J., Li, Y., Xu, Z. H., Xu, H. Y., Sun, K., & Tao, W. Y. (2006). Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology. Bioresource Technology, 97(10), 1209–1216. doi:10.1016/j.biortech.2005.05.005.
Box, G. E. P., Hunter, W. G., & Hunter, J. S. (1978). Statistics for experimenters. New York: Wiley.
Zheng, Z. M., Hu, Q. L., Hao, J., Xu, F., Guo, N. N., Sun, Y., et al. (2008). Statistical optimization of culture conditions for 1, 3-propanediol by Klebsiella pneumoniae AC 15 via central composite design. Bioresource Technology, 99(5), 1052–1056. doi:10.1016/j.biortech.2007.02.038.
Varga, E., & Klinke, H. B. (2004). High solid simultaneous saccharification and fermentation of wet oxidized corn stover to ethanol. Biotechnology and Bioengineering, 88, 67–68. doi:10.1002/bit.20222.
O’Dwyer, J. P., Zhu, L., & Granda, C. B. (2007). Enzymatic hydrolysis of lime-pretreated corn stover and investigation of the HCH-1 Model: inhibition pattern, degree of inhibition, validity of simplified HCH-1 Model. Bioresource Technology, 98, 2969–2977. doi:10.1016/j.biortech.2006.10.014.
Öhgren, K., & Bura, R. (2007). A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochemistry, 42, 834–839. doi:10.1016/j.procbio.2007.02.003.
Öhgren, K., & Rudolf, A. (2006). Fuel ethanol production from steam-pretreated corn stover using SSF at higher dry matter content. Biomass and Bioenergy, 30, 863–869. doi:10.1016/j.biombioe.2006.02.002.
Ballesteros, M., Oliva, J. M., Manzanares, P., Negro, M. J., & Ballesteros, I. (2002). Ethanol production from paper material using a simultaneous saccharification and fermentation system in a fed-batch basis. World Journal of Microbiology & Biotechnology, 18, 559–561. doi:10.1023/A:1016378326762.
Acknowledgements
This study was supported by the National Basic Research of China (2004CB719703) and State 863 Program (2007AA100702-3).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, Q., Cheng, Kk., Zhang, Ja. et al. Statistical Optimization of Recycled-Paper Enzymatic Hydrolysis for Simultaneous Saccharification and Fermentation Via Central Composite Design. Appl Biochem Biotechnol 160, 604–612 (2010). https://doi.org/10.1007/s12010-008-8446-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8446-2