Abstract
The purpose of this paper is to investigate the protein enrichment of apple pomace by Gongronella butleri through solid-state cultivation and addition of this material as feed for tilapia fry (Oreochromis niloticus). Factorial experimental design was used for the assessment of culture conditions to determine the effects of the source of nitrogen, initial moisture, and granulometry on the protein enrichment of apple pomace. During culture, the consumption of reducing sugars and the production of soluble protein were determined. The best conditions obtained were with urea (5% w/w), initial moisture of 70% and granulometry in the range from 0.85 to 1.70 mm, producing 19.63% of soluble protein. The fry submitted to the diet containing treated apple pomace presented an increase of 44% in body mass, demonstrating that apple pomace biotransformed may represent an excellent food supplement.
Similar content being viewed by others
References
Anupama, R., & Ravindra, P. (2000). Biotechnology Advances, 18, 459–479.
Singh, A., Abidi, A. B., Agarwal, A. K., & Dharmwal, N. S. (1998). Zentralbl Microbiol, 3, 149–181.
El-Saadany, R., Khalaf, H., El-Manawaty, H., & Salom, F. (1988). Acta-Alimentus, 17, 376–377.
Kolani, S., Delgenes, J. P., Moletta, R., Traore, Q., & Doh, A. (1996). Bioresource Technology, 57, 275–281.
Kim, J. H., & Lebeault, J. M. (1981). European Journal of Applied Microbiology and Biotechnology, 13, 151–154.
Zadrazil, F., & Puniya, A. K. (1995). Bioresource Technology, 54, 85–87.
Del Bianchi, V. L., Moraes, I. O., & Capalbo, D. M. F. (2001) In Schmidell, W., et al. (Eds.), Biotecnologia industrial, vol. 2: Fermentação em Estado Sólido (pp. 247–270). São Paulo, Brasil: Edgard Blücher.
Martins, E. S., Silva, D., Da Silva, R., & Gomes, E. (2002). Process Biochemistry, 37, 949–954.
Balasubramaniem, A. K., Nagarajan, K. V., & Paramasamy, G. (2001). Process Biochemistry, 36, 1241–1247.
Jecu, L. (2000). Industrial Crops and Products, 11, 1–5.
Benjamin, S., & Pandey, A. (2000). Brazilian Archives of Biology and Technology, 43(5), 453–460.
Ghanem, N. B., Yusef, H. H., & Mahrouse, H. K. (2000). Bioresource Technology, 73, 113–121.
Ikasari, L., & Mitchell, D. A. (1996). Enzyme and Microbial Technology, 19, 171–175.
ABPM-Associação Brasileira dos Produtores de Maçãs. Available from: http://www.abpm.org.br. Accessed August 22, 2007.
Berovic, M., & Ostroversnik, H. (1997). Journal of Biotechnology, 53, 47–53.
Albuquerque, P. M., Koch, F., Trossini, T. G., Esposito, E., & Ninow, J. L. (2006). Brazilian Archives of Biology and Technology, 49, 91–100.
Villas-Bôas, S. G., & Esposito, E. (2000). Biotecnologia, Ciência & Desenvolvimento, 14, 38–42.
Villas-Bôas, S. G., Esposito, E., & Mitchell, D. A. (2002). Journal of Animal Feed Science and Technology, 98, 1–12.
Foo, L. Y., & Lu, Y. (1999). Food Chemistry, 64, 511–518.
Zheng, Z., & Shetty, K. (1998). Journal of Agricultural and Food Chemistry, 46, 783–787.
Zheng, Z., & Shetty, K. (2000). Process Biochemistry, 36, 79–84.
Jin, H., Kim, H. S., Kim, S. K., Shin, M. K., Kim, J. H., & Lee, J. W. (2002). Enzyme and Microbial Technology, 30, 822–827.
Vendruscolo, F., Koch, F., Pitol, L. O., & Ninow, J. L. (2007). Revista Brasileira de Tecnologia Agroindustrial, 1, 53–57.
Favela-Torres, E., Volke-Sepulveda, T., & Viniegra-Gonzalvez, G. (2006). Production of hydrolytic depolymerising pectinases. Food Technology and Biotechnology, 44, 221–227.
Shrikot, C. K., Sharma, N., & Sharma, S. (2004). Apple pomace: An alternative substrate for xylanase production by na alkalophilic Bacillus macerans by using solid-state fermentation. Journal of Microbial World, 6, 20–26.
Shojaosadati, S. A., & Babaeipour, V. (2002). Citric acid production from apple pomace in multi-layer packed bed solid-state bioreactor. Process Biochemistry, 37, 909–914.
Bhalla, T. C., & Joshi, M. (1994). Protein enrichment of apple pomace by co-culture of cellulolytic moulds and yeasts. World Journal of Microbiology & Biotechnology, 10, 116–117.
Devrajan, A., Joshi, V. K., Gupta, K., Sheikher, C., & Lal, B. B. (2004). Evaluation of apple pomace based reconstituted feed in rats after solid state fermentation and ethanol recovery. Brazilian Archives of Biology and Technology, 47, 93–106.
Zheng, Z., & Shetty, K. (2000). Solid state production of polygalacturonase by Lentinus edodes using fruit processing wastes. Process Biochemistry, 35, 825–830.
Worral, J. J., & Yang, C. S. (1992). Shiitake and oyster mushroom production on apple pomace and sawdust. HortScience, 27, 1131–1133.
Ngadi, M. O., & Correia, L. R. (1992a). Kinetics of solid state ethanol fermentation from apple pomace. Journal of Food Engineering, 17, 97–116.
Ngadi, M. O., & Correia, L. R. (1992b). Solid state ethanol fermentation of apple pomace as affected by moisture and bioreactor mixing speed. Journal of Food Science, 57, 667–670.
Paganini, C., Nogueira, A., Silva, N. C., & Wosiacki, G. (2005). Utilization of apple pomace for ethanol production and food fiber obtainment. Ciência Agrotécnica, Lavras, 29, 1231–1238.
Bramorski, A., Soccol, C. R., Christen, P., & Revah, S. (1998). Fruit aroma production by Ceratocystis fimbriata in solid cultures from agroindustrial wastes. Revista de Microbiologia (online), 29. Available from World Wide Web:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S000137141998000300012& lng=en&nrm=iso>.
Tsurumi, R., Shiraishi, S., Ando, Y., Yanagida, M., & Takeda, K. (2001). Production of flavor compounds from apple pomace. Nippon Shokuhin Kagaku Kogaku Kaishi, 48, 564–569.
Medeiros, A. B. P., Pandey, A., Vandenberghe, L. P. S., Pastore, G. M., & Soccol, C. R. (2006). Production and recovery of aroma compounds produced by solid-state fermentation using different adsorbents. Food Technology and Biotechnology, 44, 47–51.
Foo, L. Y., & Lu, Y. (1999). Isolation and identification of procyanidins in apple pomace. Food Chemistry, 64, 511–518.
Lu, Y., & Foo, L. Y. (2000). Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry, 68, 81–85.
Grigelmo-Miguel, N., & Martín-Belloso, O. (1999). Comparison of dietary fibre from by-products of processing fruits and greens and from cereals. LWT-Food Science and Technology, 32, 503–508.
Masoodi, F. A., Sharma, B., & Chauhan, G. S. (2002). Use of apple pomace as a source of dietary fiber in cakes. Plant Foods for Human Nutrition, 57, 121–128.
El-Sayed, A. F. M. (1999). Aquaculture, 179, 149–168.
Cavalheiro, J. M. O., Souza, E. O., & Bora, P. S. (2007). Bioresource Technology, 98, 602–606.
Sabra, G. E. (2004). Master’s degree dissertation. Universidade de Mogi das Cruzes, Mogi das Cruzes, BR.
Ulloa Rojas, J. B., & Verreth, J. A. J. (2003). Aquaculture, 217, 275–283.
Dabrowski, M., El-Saidy, A. F. M., & Wisner, N. (2002). Aquaculture Nutrition, 7, 189–195.
Dongmeza, E., Siddhuraju, P., Francis, G., & Becker, K. (2006). Aquaculture, 261, 407–422.
Novoa, M. A. O., Palacios, C. A. M., & Castilho, L. O. (2002). Aquaculture Nutrition, 8, 257–263.
Abdolsamad, K. A., Verreth, J. A. J., & Schrama, J. W. (2006). Aquaculture, 260, 194–205.
Lowry, O. H., Rosebrough, N. J., & Farr, A. L. (1951). Journal of Biological Chemistry, 193, 265–275.
Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.
AOAC (1995). Association of official analytical chemists. Ed—Official Methods of Analysis (16th Ed.). Washington, 1094 p.
APHA (1995). Standard methods for the examination of water and wastewater (19th Ed.). Washington: American Public Health Association.
Bisaria, R., Madan, M., & Vasudevan, P. (1997). Bioresource Technology, 59, 5–8.
Baccarin, A. E., & Pezzato, L. E. (2001). Pesquisa Agropecuaâria Brasileira, 36, 549–556.
Davies, S., & Wareham, H. (1988). Aquaculture, 73, 189–199.
Mahnken, C. V. W., Spinelli, J., & Waknitz, F. W. (1980). Aquaculture, 20, 41–56.
Acknowledgements
The authors wish to thank the Fundacão de Amparo à Pesquisa do Estado de São Paulo (FAPESP-Foundation for the Support of Research in the State of São Paulo, Process Number 05/55837-6) for financial support, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-The National Council for Scientific and Technological Development) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Coordination for Profesional Enrichment for Higher Education) for research scholarships, and the company Yakult S.A. Finally, we wish to thank Robert Samohyl for technical support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Vendruscolo, F., da Silva Ribeiro, C., Esposito, E. et al. Protein Enrichment of Apple Pomace and Use in Feed for Nile Tilapia. Appl Biochem Biotechnol 152, 74–87 (2009). https://doi.org/10.1007/s12010-008-8259-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8259-3