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Protein Enrichment of Apple Pomace and Use in Feed for Nile Tilapia

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Abstract

The purpose of this paper is to investigate the protein enrichment of apple pomace by Gongronella butleri through solid-state cultivation and addition of this material as feed for tilapia fry (Oreochromis niloticus). Factorial experimental design was used for the assessment of culture conditions to determine the effects of the source of nitrogen, initial moisture, and granulometry on the protein enrichment of apple pomace. During culture, the consumption of reducing sugars and the production of soluble protein were determined. The best conditions obtained were with urea (5% w/w), initial moisture of 70% and granulometry in the range from 0.85 to 1.70 mm, producing 19.63% of soluble protein. The fry submitted to the diet containing treated apple pomace presented an increase of 44% in body mass, demonstrating that apple pomace biotransformed may represent an excellent food supplement.

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References

  1. Anupama, R., & Ravindra, P. (2000). Biotechnology Advances, 18, 459–479.

    Article  CAS  Google Scholar 

  2. Singh, A., Abidi, A. B., Agarwal, A. K., & Dharmwal, N. S. (1998). Zentralbl Microbiol, 3, 149–181.

    Google Scholar 

  3. El-Saadany, R., Khalaf, H., El-Manawaty, H., & Salom, F. (1988). Acta-Alimentus, 17, 376–377.

    Google Scholar 

  4. Kolani, S., Delgenes, J. P., Moletta, R., Traore, Q., & Doh, A. (1996). Bioresource Technology, 57, 275–281.

    Article  Google Scholar 

  5. Kim, J. H., & Lebeault, J. M. (1981). European Journal of Applied Microbiology and Biotechnology, 13, 151–154.

    Article  CAS  Google Scholar 

  6. Zadrazil, F., & Puniya, A. K. (1995). Bioresource Technology, 54, 85–87.

    Article  CAS  Google Scholar 

  7. Del Bianchi, V. L., Moraes, I. O., & Capalbo, D. M. F. (2001) In Schmidell, W., et al. (Eds.), Biotecnologia industrial, vol. 2: Fermentação em Estado Sólido (pp. 247–270). São Paulo, Brasil: Edgard Blücher.

  8. Martins, E. S., Silva, D., Da Silva, R., & Gomes, E. (2002). Process Biochemistry, 37, 949–954.

    Article  CAS  Google Scholar 

  9. Balasubramaniem, A. K., Nagarajan, K. V., & Paramasamy, G. (2001). Process Biochemistry, 36, 1241–1247.

    Article  CAS  Google Scholar 

  10. Jecu, L. (2000). Industrial Crops and Products, 11, 1–5.

    Article  CAS  Google Scholar 

  11. Benjamin, S., & Pandey, A. (2000). Brazilian Archives of Biology and Technology, 43(5), 453–460.

    Article  CAS  Google Scholar 

  12. Ghanem, N. B., Yusef, H. H., & Mahrouse, H. K. (2000). Bioresource Technology, 73, 113–121.

    Article  CAS  Google Scholar 

  13. Ikasari, L., & Mitchell, D. A. (1996). Enzyme and Microbial Technology, 19, 171–175.

    Article  CAS  Google Scholar 

  14. ABPM-Associação Brasileira dos Produtores de Maçãs. Available from: http://www.abpm.org.br. Accessed August 22, 2007.

  15. Berovic, M., & Ostroversnik, H. (1997). Journal of Biotechnology, 53, 47–53.

    Article  CAS  Google Scholar 

  16. Albuquerque, P. M., Koch, F., Trossini, T. G., Esposito, E., & Ninow, J. L. (2006). Brazilian Archives of Biology and Technology, 49, 91–100.

    Article  CAS  Google Scholar 

  17. Villas-Bôas, S. G., & Esposito, E. (2000). Biotecnologia, Ciência & Desenvolvimento, 14, 38–42.

    Google Scholar 

  18. Villas-Bôas, S. G., Esposito, E., & Mitchell, D. A. (2002). Journal of Animal Feed Science and Technology, 98, 1–12.

    Article  Google Scholar 

  19. Foo, L. Y., & Lu, Y. (1999). Food Chemistry, 64, 511–518.

    Article  CAS  Google Scholar 

  20. Zheng, Z., & Shetty, K. (1998). Journal of Agricultural and Food Chemistry, 46, 783–787.

    Article  CAS  Google Scholar 

  21. Zheng, Z., & Shetty, K. (2000). Process Biochemistry, 36, 79–84.

    Article  CAS  Google Scholar 

  22. Jin, H., Kim, H. S., Kim, S. K., Shin, M. K., Kim, J. H., & Lee, J. W. (2002). Enzyme and Microbial Technology, 30, 822–827.

    Article  CAS  Google Scholar 

  23. Vendruscolo, F., Koch, F., Pitol, L. O., & Ninow, J. L. (2007). Revista Brasileira de Tecnologia Agroindustrial, 1, 53–57.

    Google Scholar 

  24. Favela-Torres, E., Volke-Sepulveda, T., & Viniegra-Gonzalvez, G. (2006). Production of hydrolytic depolymerising pectinases. Food Technology and Biotechnology, 44, 221–227.

    CAS  Google Scholar 

  25. Shrikot, C. K., Sharma, N., & Sharma, S. (2004). Apple pomace: An alternative substrate for xylanase production by na alkalophilic Bacillus macerans by using solid-state fermentation. Journal of Microbial World, 6, 20–26.

    CAS  Google Scholar 

  26. Shojaosadati, S. A., & Babaeipour, V. (2002). Citric acid production from apple pomace in multi-layer packed bed solid-state bioreactor. Process Biochemistry, 37, 909–914.

    Article  CAS  Google Scholar 

  27. Bhalla, T. C., & Joshi, M. (1994). Protein enrichment of apple pomace by co-culture of cellulolytic moulds and yeasts. World Journal of Microbiology & Biotechnology, 10, 116–117.

    Article  CAS  Google Scholar 

  28. Devrajan, A., Joshi, V. K., Gupta, K., Sheikher, C., & Lal, B. B. (2004). Evaluation of apple pomace based reconstituted feed in rats after solid state fermentation and ethanol recovery. Brazilian Archives of Biology and Technology, 47, 93–106.

    Article  Google Scholar 

  29. Zheng, Z., & Shetty, K. (2000). Solid state production of polygalacturonase by Lentinus edodes using fruit processing wastes. Process Biochemistry, 35, 825–830.

    Article  CAS  Google Scholar 

  30. Worral, J. J., & Yang, C. S. (1992). Shiitake and oyster mushroom production on apple pomace and sawdust. HortScience, 27, 1131–1133.

    Google Scholar 

  31. Ngadi, M. O., & Correia, L. R. (1992a). Kinetics of solid state ethanol fermentation from apple pomace. Journal of Food Engineering, 17, 97–116.

    Article  Google Scholar 

  32. Ngadi, M. O., & Correia, L. R. (1992b). Solid state ethanol fermentation of apple pomace as affected by moisture and bioreactor mixing speed. Journal of Food Science, 57, 667–670.

    Article  CAS  Google Scholar 

  33. Paganini, C., Nogueira, A., Silva, N. C., & Wosiacki, G. (2005). Utilization of apple pomace for ethanol production and food fiber obtainment. Ciência Agrotécnica, Lavras, 29, 1231–1238.

    CAS  Google Scholar 

  34. Bramorski, A., Soccol, C. R., Christen, P., & Revah, S. (1998). Fruit aroma production by Ceratocystis fimbriata in solid cultures from agroindustrial wastes. Revista de Microbiologia (online), 29. Available from World Wide Web:<http://www.scielo.br/scielo.php?script=sci_arttext&pid=S000137141998000300012& lng=en&nrm=iso>.

  35. Tsurumi, R., Shiraishi, S., Ando, Y., Yanagida, M., & Takeda, K. (2001). Production of flavor compounds from apple pomace. Nippon Shokuhin Kagaku Kogaku Kaishi, 48, 564–569.

    CAS  Google Scholar 

  36. Medeiros, A. B. P., Pandey, A., Vandenberghe, L. P. S., Pastore, G. M., & Soccol, C. R. (2006). Production and recovery of aroma compounds produced by solid-state fermentation using different adsorbents. Food Technology and Biotechnology, 44, 47–51.

    CAS  Google Scholar 

  37. Foo, L. Y., & Lu, Y. (1999). Isolation and identification of procyanidins in apple pomace. Food Chemistry, 64, 511–518.

    Article  CAS  Google Scholar 

  38. Lu, Y., & Foo, L. Y. (2000). Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry, 68, 81–85.

    Article  CAS  Google Scholar 

  39. Grigelmo-Miguel, N., & Martín-Belloso, O. (1999). Comparison of dietary fibre from by-products of processing fruits and greens and from cereals. LWT-Food Science and Technology, 32, 503–508.

    CAS  Google Scholar 

  40. Masoodi, F. A., Sharma, B., & Chauhan, G. S. (2002). Use of apple pomace as a source of dietary fiber in cakes. Plant Foods for Human Nutrition, 57, 121–128.

    Article  Google Scholar 

  41. El-Sayed, A. F. M. (1999). Aquaculture, 179, 149–168.

    Article  Google Scholar 

  42. Cavalheiro, J. M. O., Souza, E. O., & Bora, P. S. (2007). Bioresource Technology, 98, 602–606.

    Article  Google Scholar 

  43. Sabra, G. E. (2004). Master’s degree dissertation. Universidade de Mogi das Cruzes, Mogi das Cruzes, BR.

  44. Ulloa Rojas, J. B., & Verreth, J. A. J. (2003). Aquaculture, 217, 275–283.

    Article  Google Scholar 

  45. Dabrowski, M., El-Saidy, A. F. M., & Wisner, N. (2002). Aquaculture Nutrition, 7, 189–195.

    Google Scholar 

  46. Dongmeza, E., Siddhuraju, P., Francis, G., & Becker, K. (2006). Aquaculture, 261, 407–422.

    Article  Google Scholar 

  47. Novoa, M. A. O., Palacios, C. A. M., & Castilho, L. O. (2002). Aquaculture Nutrition, 8, 257–263.

    Article  Google Scholar 

  48. Abdolsamad, K. A., Verreth, J. A. J., & Schrama, J. W. (2006). Aquaculture, 260, 194–205.

    Article  Google Scholar 

  49. Lowry, O. H., Rosebrough, N. J., & Farr, A. L. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  50. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  51. AOAC (1995). Association of official analytical chemists. Ed—Official Methods of Analysis (16th Ed.). Washington, 1094 p.

  52. APHA (1995). Standard methods for the examination of water and wastewater (19th Ed.). Washington: American Public Health Association.

    Google Scholar 

  53. Bisaria, R., Madan, M., & Vasudevan, P. (1997). Bioresource Technology, 59, 5–8.

    Article  CAS  Google Scholar 

  54. Baccarin, A. E., & Pezzato, L. E. (2001). Pesquisa Agropecuaâria Brasileira, 36, 549–556.

    Google Scholar 

  55. Davies, S., & Wareham, H. (1988). Aquaculture, 73, 189–199.

    Article  CAS  Google Scholar 

  56. Mahnken, C. V. W., Spinelli, J., & Waknitz, F. W. (1980). Aquaculture, 20, 41–56.

    Article  Google Scholar 

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Acknowledgements

The authors wish to thank the Fundacão de Amparo à Pesquisa do Estado de São Paulo (FAPESP-Foundation for the Support of Research in the State of São Paulo, Process Number 05/55837-6) for financial support, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-The National Council for Scientific and Technological Development) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Coordination for Profesional Enrichment for Higher Education) for research scholarships, and the company Yakult S.A. Finally, we wish to thank Robert Samohyl for technical support.

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Correspondence to Jorge Luiz Ninow.

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Vendruscolo, F., da Silva Ribeiro, C., Esposito, E. et al. Protein Enrichment of Apple Pomace and Use in Feed for Nile Tilapia. Appl Biochem Biotechnol 152, 74–87 (2009). https://doi.org/10.1007/s12010-008-8259-3

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