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Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1

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Abstract

Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient ‘R’ was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.

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Acknowledgments

Authors are thankful to the Department of Biotechnology, Punjabi University, Patiala for providing the necessary laboratory facilities for this work. The financial assistance received under the FIST program from Department of Science and Technology, Govt. of India, New Delhi is duly acknowledged.

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Authors and Affiliations

  1. Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002, Punjab, India

    Ram Sarup Singh, Harpreet Singh & Gaganpreet Kaur Saini

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  1. Ram Sarup Singh
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Correspondence to Ram Sarup Singh.

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Singh, R.S., Singh, H. & Saini, G.K. Response Surface Optimization of the Critical Medium Components for Pullulan Production by Aureobasidium pullulans FB-1. Appl Biochem Biotechnol 152, 42–53 (2009). https://doi.org/10.1007/s12010-008-8180-9

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