Abstract
One of the major wastes generated by fertilizer, explosive, and nuclear industries are nitrate (as high as 1,000 ppm NO3N) whose removal before disposal has become a growing concern. In this study, an active denitrifying sludge was immobilized onto support materials like cloth and polyurethane foam and their denitrification efficiency on high nitrate wastes [1,000 ppm NO3 (225 ppm NO3N), 5,000 ppm NO3 (1,129 ppm NO3N), 7,500 ppm NO3 (1,693 ppm NO3 N)] was studied. Results showed complete degradation of the nitrate wastes (225 ppm NO3N, 1,129 ppm NO3N, and 1,693 ppm NO3N) without any accumulation of nitrite in a period of only 1, 4, and 10 h, respectively. Based on adhering and entrapment principle, an immobilization unit was developed using a combination of cloth and foam as well as both individually. This system used for treating such high nitrate wastes was found to be quite effective in waste water treatment, particularly in problems associated with solid–liquid separation. The batch column reactor was run in about 45 batches without any loss in activity or reactor stability.
Similar content being viewed by others
References
Bouchard, D. C., Williams, M. K., & Surampalli, R. Y. (1992). Journal of the American Water Works Association, 84, 85–90.
Young, J. C., & Yang, B. S. (1989). Pollution Control Federation, 61, 1576–1587.
Albagnae, G. (1990). Water Science and Technology, 22, 17–24.
Cheng, S. S., Huang, S. Y., Lay, J. J., Tsai, P. S., & Cho, L. T. (1992). Water Science and Technology, 26, 503–510.
Camargo, S. A. R., & Nour, E. A. A. (2001). Water Science and Technology, 44, 63–70.
Verrier, D., Mortier, B., & Albagnae, G. (1987). Biotechnology Letters, 10, 735–740.
Vandevivere, P., & Kirchman, D. L. (1993). Applied Environmental and Microbiology, 59, 3280–3286.
Picanco, A. P., Vallero, M. V. G., Gianotti, E. P., Zaiat, M., & Blundi, C. E. (2001). Water Science and Technology, 44, 197–204.
Huysman, P., van Meneen, P., van Assche, P., & Verstraete, W. (1983). Biotechnology Letters, 5, 643–648.
Calzada, J. F., Arriola, M. C., Castañeda, J. E., Godoy, J. E., & Rolz, C. (1984). Biotechnology Letters, 6, 385–388.
Gizgen, H. J., Schoenmakers, T. J. M., Caerteling, C. G. M., & Vogels, G. D. (1988). Biotechnology Letters, 10, 61–66.
Zaiat, M., Vieira, L. G. T., & Foresti, E. (1997). Water Research, 31, 1760–1766.
Havens, P. L., & Howard, F. R. (1993). Industrial & Engineering Chemistry Research, 32, 2254–2258.
Varesche, M. B., Zaiat, M., Vieira, L. G. T., Vazoller, R. F., & Foresti, E. (1997). Applied Microbiology and Biotechnology, 48, 534–538.
D’Souza, S. F., & Kamath, N. (1988). Applied Microbiology and Biotechnology, 29, 136–140.
Joshi, S., & Yamazaki, H. (1984). Biotechnology Letters, 6, 797–802.
D’Souza, S. F., & Kubal, B. S. (2002). Journal of Biochemical and Biophysical Methods, 51, 151–159.
Lewis, V. P., & Yang, S. T. (1992). Biotechnology and Bioengineering, 40, 465–474.
D’Souza, S. F., & Melo, J. S. (2001). Process Biochemistry, 36, 677–681.
Dhamole, P. B., Nair, R. R., D’Souza, S. F., & Lele, S. S. (2007). Bioresource Technology, 98, 247–252.
Nair, R. R., Dhamole, P. B., Lele, S. S., & D’Souza, S. F. (2007). Chemosphere, 67, 1612–1617.
D’Souza, S. F., & Kamath, N. (1991). Enzyme and Microbial Technology, 13, 935–938.
Marshall, R. C. (1984). In Microbial adhesion and aggregation. New York: Springer.
Acknowledgments
The authors thank Department of Atomic Energy (India) for funding this work and Rashtriya Chemicals and Fertilizers, Mumbai (India) for providing the sludge.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nair, R.R., Dhamole, P.B., Lele, S.S. et al. Biotreatment of High Strength Nitrate Waste Using Immobilized Preadapted Sludge. Appl Biochem Biotechnol 151, 193–200 (2008). https://doi.org/10.1007/s12010-008-8170-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8170-y