Abstract
This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viability and protein secretion between free and immobilized cells. After 12 h, microcapsules with a cell density of 4.8 × 105 colony forming unit (CFU) ml−1 grew to 2.2 × 108 CFU ml−1 and released 0.24 arbitrary unit (AU) ml−1 of nuclease, producing similar results as free cells, which grew from 3.4 × 105 to 1.9 × 108 CFU ml−1 and secreted 0.21 AU ml−1 of nuclease. Moreover, encapsulated cells at a density of 4.4 × 107 CFU ml−1 grew to 2.2 × 1010 CFU ml−1 in 12 h and secreted 15.3 AU ml−1 of nuclease although 3.1 × 107 CFU ml−1 of free cells reached only 2.3 × 109 CFU ml−1 and released 5.6 AU ml−1 of nuclease. We also show the sustained stability of the microcapsules during storage at 4°C over 8 weeks.
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Acknowledgements
We sincerely thank Phillipe Langella for provision of L. lactis cells. This work was supported by research grants provided to Dr. Prakash from the Canadian Institute of Health Research.
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Lawuyi, B., Chen, H., Afkhami, F. et al. Microencapsulated Engineered Lactococcus lactis Cells for Heterologous Protein Delivery: Preparation and In Vitro Analysis. Appl Biochem Biotechnol 142, 71–80 (2007). https://doi.org/10.1007/s12010-007-0002-y
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DOI: https://doi.org/10.1007/s12010-007-0002-y