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Characterization of Rice Protein Hydrolysate/Chitosan Composite Films and Their Bioactivities Evaluation When Incorporating Curcumin: Effect of Genipin Concentration

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Abstract

The effect of genipin (GP) concentrations on the structural, mechanical, and water barrier properties of chitosan (CS)/rice protein hydrolysate (RH) composite films was investigated for further incorporating of curcumin. GP increased the stability of CS/RH conjugate in solution. The degree of CS/RH crosslinking depended on genipin concentration, as indicated in particle size and UV spectra. At optimal genipin concentration (0.4 mg GP/g total biopolymers), the CS/RH film had the lowest moisture content (10.67%) and water vapor transmission rate (829.35 g/m2·24 h.). Genipin could enhance the tensile strength and thermal stability of the CS/RH films depending on GP addition concentration. With an increased amount of GP, the DPPH scavenging activity decreased due to the cross-linking of antioxidant peptides, and the E* value increased indicating the significant color differences caused by increasing GP. Notably, the addition of curcumin had a positive effect on the DPPH scavenging activity and antimicrobial activity of the CS/RH films. Curcumin-loaded CS/RH films had higher antibacterial activity against S. aureus than E. coli. In general, the suitable addition content of GP was a promising and green strategy to improve the properties of the CS/RH films.

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Funding

The present work was sponsored by the National Natural Science Foundation of China (31860451) and the Natural Science Fund for Distinguished Young Scholars (20192BCB23006) of Jiangxi Province.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, Jiangxi, China

    Hexiang Xie, Liqiong Zhang, Kefan Ouyang, Hua Xiong & Qiang Zhao

  2. School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia

    Yong Wang

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  1. Hexiang Xie
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  2. Liqiong Zhang
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  4. Yong Wang
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Correspondence to Qiang Zhao.

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Xie, H., Zhang, L., Ouyang, K. et al. Characterization of Rice Protein Hydrolysate/Chitosan Composite Films and Their Bioactivities Evaluation When Incorporating Curcumin: Effect of Genipin Concentration. Food Bioprocess Technol 16, 2159–2171 (2023). https://doi.org/10.1007/s11947-023-03056-7

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