Abstract
Cereal flakes are the most popular breakfast choices in the segment of ready-to-eat food products and generally consumed with hot milk. Fortification of cereal flakes with probiotics may increase the value addition, but the high temperature of milk limits the use of live microorganisms. The present work aims to develop the thermo-stable probiotic cereal flakes, which can withstand in hot milk up to 80 °C temperature. A coating blend containing Saccharomyces boulardii (109 CFU/mL) and gum Arabic (6% w/v) was applied on various cereal flakes, and the survivability was evaluated in the hot milk. Cornflakes showed better compatibility with the coating mixture by showing maximum survivability of S. boulardii (7.30 log CFU/g) over the control (2.61 log CFU/g). Further, the addition of nutraceuticals such as trehalose, glutathione, and oryzanol in different concentrations to coating mixture was also assessed. The cornflakes containing trehalose (0.4 mg/g) in its coating have resulted in maximum survivability of 7.99 log CFU/g and showed 3.26-fold higher thermo-protective role as compared to control. Sensory analysis showed an increased acceptability of gum Arabic coated cornflakes over the control due to change in viscosity of milk after its addition. SEM analysis clearly differentiated the surface based on treatments given to cornflakes. Modified atmosphere packaging of probiotic cornflakes using a vacuum, nitrogen gas, and air was done and then storage studies were done for 90 days.
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Abbreviations
- CAGR:
-
Compound annual growth rate
- CF:
-
Toasted cornflakes (uncoated)
- CF + GA:
-
Toasted cornflakes coated using 6% gum Arabic solution
- CF + SB:
-
Toasted cornflakes coated with S. boulardii solution without using gum Arabic
- CF + SB + GA:
-
Toasted cornflakes coated with S. boulardii and 6% gum Arabic solution
- CF + SB + GA + Tre:
-
Toasted cornflakes coated with S. boulardii, 6% gum Arabic solution, and trehalose 0.4 mg/g of cornflakes
- CFU:
-
Colony forming units
- FAO:
-
Food and Agriculture Organization of the United Nations
- FEG-SEM:
-
Field emission gun-scanning electron microscopy
- FSSAI:
-
Food Safety and Standards Authority of India
- GA:
-
Gum Arabic
- GUT:
-
Genitourinary tract
- MAP:
-
Modified atmosphere packaging
- RPM:
-
Revolution per minute
- US-FDA:
-
United State Food and Drug Administration
- YPD:
-
Yeast extract, peptone, and dextrose
- SEM:
-
Scanning electron microscopy
- Tre:
-
Trehalose
- WHO:
-
World Health Organization
- WI:
-
Whiteness index
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Acknowledgments
The authors would like to thank the sensory panelists for giving their valuable time and suggestions.
Funding
The research work was supported by the University Grant Commission, Government of India under the BSR scheme, and the Institute of Chemical Technology, Mumbai, India, for availing all the required facilities for the research work.
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Singu, B.D., Bhushette, P.R. & Annapure, U.S. Survivability Assessment of Saccharomyces boulardii in a Symbiotic System Using Nutraceuticals and Modified Atmosphere Packaging. Food Bioprocess Technol 13, 693–704 (2020). https://doi.org/10.1007/s11947-020-02430-z
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DOI: https://doi.org/10.1007/s11947-020-02430-z