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Microbiological Profile of Goat Meat Inoculated with Lactic Acid Bacteria Cultures and Stored at 30°C for 7 days

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Abstract

The ability of lactic acid bacteria (LAB) cultures to preserve goat meat at 30°C was evaluated in the present study. Strains of Pediococcus pentosaceus GOAT 01 and Lactobacillus plantarum GOAT 012, individually and in combination, were applied as starters on sliced meat samples at 6 log cfu/g and stored for 7 days at 30°C to simulate ambient temperature in Nigeria. They were evaluated for microbiological profile during storage. Reduction in bacterial counts was recorded for enterobacteriaceae, Staphylococcus, yeasts and moulds in starter culture inoculated samples (SCIS), whereas an increase occurred in uninoculated control samples (UCS). In challenge experimental trials, two different sets of meat were inoculated individually with 6 log cfu/g each of pathogenic organisms, Listeria monocytogenes and Salmonella Typhimurium. The inoculated pathogens were monitored during storage to assess the influence of starter cultures on them. Approximately 1 log reduction was recorded in the viable count of L. monocytogenes on day 1, while counts were below detection limit (<2 log) on day 2 in meat samples inoculated with P. pentosaceus alone and in combination with L. plantarum. Counts of Salmonella Typhimurium showed about 2 log reduction in SCIS, inoculated with P. pentosaceus alone and in combination with L. plantarum, on day 2 while an increase by 4 logs was observed in UCS. Our findings suggest that the protective effect of the LAB strains could be exploited in shelf life extension and control of foodborne pathogens in goat meat. If the starter strains could be improved upon, their potential as biopreservatives may be engaged in the preservation of the meat in Nigeria, where storage systems have been very inadequate.

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References

  • Albano, H., Todorov, S. D., Reenen, C. A., Hogg, T., Dicks, L. M. T., & Teixeira, P. (2007). Characterization of two bacteriocins produced by Pediococcus acidilactici isolated from “Alheira”, a fermented sausage traditionally produced in Portugal. International Journal of Food Microbiology, 116, 239–247.

    Article  CAS  Google Scholar 

  • Aymerich, M. T., Hugas, M., & Monfort, J. M. (1998). Review: bacteriocinogenic lactic acid bacteria associated with meat products. Food Science and Technology International, 4, 141–158.

    Article  Google Scholar 

  • Bulut, C., Gunes, H., Okuklu, B., Harsa, S., Kilic, S., Coban, H. S., et al. (2005). Homofermentative lactic acid bacteria of a traditional cheese, Comlek peyniri from Cappadocia region. Journal of Dairy Research, 72, 19–24.

    Article  CAS  Google Scholar 

  • Calo-Mata, P., Arlindo, S., Boehme, K., de Miguel, T., Pascoal, A., & Barros-Velazquez, J. (2008). Current applications and future trends of lactic acid bacteria and their bacteriocins for the biopreservation of aquatic food products. Food Bioprocess and Technology, 1, 43–63.

    Article  Google Scholar 

  • Casaburi, A., Aristoy, M. C., Cavella, S., Di Monaco, R., Ercolini, D., Toldra, F., et al. (2007). Biochemical and sensory characteristics of traditional fermented sausages of Vallo di Diano (Southern Italy) as affected by the use of starter cultures. Meat Science, 76, 295–307.

    Article  CAS  Google Scholar 

  • Cintas, L. M., Casaus, M. P., Herranz, C., Nes, I. F., & Hernández, P. E. (2001). Review: bacteriocins of lactic acid bacteria. Food Science and Technology International, 7, 281–305.

    CAS  Google Scholar 

  • Colak, H., Hampikyan, H., Ulusoy, B., & Bingol, E. B. (2007). Presence of L. monocytogenes in Turkish style fermented sausage (sucuk). Food Control, 18, 576–579.

    Article  Google Scholar 

  • Djenane, D., Martínez, L., Blanco, D., Yangüela, J., Beltrán, J. A., & Roncalés, P. (2005). Effect of lactic acid bacteria on extension of shelf life and growth of Listeria monocytogenes in beef steaks stored in CO2-rich atmosphere. Brazillian Journal of Microbiology, 36, 405–412.

    CAS  Google Scholar 

  • Ercolini, D., Moschetti, G., Blaiotta, G., & Coppola, S. (2001). Behaviour of variable V3 region from 16 S rDNA of important lactic acid bacteria in denaturing gradient gel electrophoresis. Current Microbiology, 42, 199–202.

    Article  CAS  Google Scholar 

  • Erkmen, O. (2008). Modeling the effects of sucuk production technique on Listeria monocytogenes, aerobic bacteria and lactic acid bacteria during ripening and storage. Food and Bioproducts Processing, 86, 220–226.

    Article  Google Scholar 

  • Gomółka-Pawlicka, M., Uradziński, J., & Wiszniewska, A. (2004). Effect of chosen lactic acid bacteria strains on Staphylococcus aureus in vitro as well as in meat and raw sausages. Polish Journal of Veterinary Science, 7, 251–259.

    Google Scholar 

  • Guerrero, I., Mendiolea, R., Ponce, E., & Prado, A. (1995). Inoculation of lactic acid bacteria (LAB) on meat surfaces as a means of decontamination in semi tropical conditions. Meat Science, 40, 397–411.

    Article  Google Scholar 

  • Gurira, O. Z., & Buys, E. M. (2005). Characterization and antimicrobial activity of Pediococcus species isolated from South African farm-style cheese. Food Microbiology, 22, 159–168.

    Article  CAS  Google Scholar 

  • Hammes, H. P., & Hertel, C. (1996). Selection and improvement of lactic acid bacteria used in meat and sausage fermentation. Lait, 76, 159–168.

    Article  CAS  Google Scholar 

  • HPA, Health Protection Agency (2004). Enumeration of Enterobacteriaceae by the Colony Count Technique. National Standard Method F 23 Issue 1. Available at http://www.hpa-standardmethods.org.uk/documents/food/pdf/F23.pdf.

  • Jyoti, B. D., Suresh, A. K., & Venkatesh, K. V. (2003). Diacetyl production and growth of Lactobacillus rhamnosus on multiple substrates. World Journal of Microbiology and Biotechnology, 19, 509–514.

    Article  CAS  Google Scholar 

  • Kaban, G., & Kaya, M. (2006). Effect of starter culture on growth of Staphylococcus aureus in sucuk. Food Control, 17, 797–801.

    Article  CAS  Google Scholar 

  • Lucke, F. K. (2000). Utilization of microbes to process and preserve meat. Meat Science, 56, 105–115.

    Article  Google Scholar 

  • Millette, M., Dupont, C., Shareck, F., Ruiz, M. T., Archambault, D., & Lacroix, M. (2008). Purification and identification of the pediocin produced by Pediococcus acidilactici MM33, a new human intestinal Strain. Journal of Applied Microbiology, 108, 269–275.

    Google Scholar 

  • Mor-Mur, M., & Yuste, J. (2010). Emerging bacterial pathogens in meat and poultry: an overview. Food Bioprocess and Technology, 3, 24–35.

    Article  Google Scholar 

  • Olaoye, O. A., & Onilude, A. A. (2009). Isolation and biochemical profiles of numerous strains of lactic acid producing bacteria from various parts of a domestic West African goat (Capra hircus). Australian Journal of Basic and Applied Sciences, 3, 460–466.

    CAS  Google Scholar 

  • Olaoye, O. A. & Onilude, A. A. (2010). Investigation on the potential use of biological agents in the extension of fresh beef in Nigeria. World Journal of Microbiology and Biotechnology, doi:10.1007/s11274-010-0319-5, in press.

  • Olaoye, O. A., Onilude, A. A., & Dodd, C. E. R. (2008). Identification of Pediococcus spp. from beef and evaluation of their lactic acid production in varying concentrations of different carbon sources. Advances in Natural and Applied Sciences, 2, 97–207.

    Google Scholar 

  • Onilude, A. A., Sanni, A. I., Olaoye, O. A., & Ogunbanwo, S. T. (2002). Influence of lactic cultures on the quality attributes of tsire, a West African stick meat. World Journal of Microbiology and Biotechnology, 18, 615–619.

    Article  CAS  Google Scholar 

  • SPSS. (1999). SPSS 10.01 for Windows. USA: SPSS.

    Google Scholar 

  • Suwanjinda, D., Eames, C., & Panbangred, W. (2007). Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods. Biochemistry and Molecular Biology Education, 35, 364–369.

    Article  CAS  Google Scholar 

  • Villegas, E., & Gilliland, S. E. (1998). Hydrogen peroxide production by Lactobacillus delbrueckii Subsp. Lactis I at 5°C. Journal of Food Science, 63, 1070–1074.

    Article  CAS  Google Scholar 

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Acknowledgement

The authors wish to thank the School of Biosciences, University of Nottingham, United Kingdom, where some aspects of this study were carried out.

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Authors and Affiliations

  1. Food Technology Department, The Federal Polytechnic, PMB 420, Offa, Kwara State, Nigeria

    Olusegun A. Olaoye & Oyeyemi A. Idowu

  2. Microbial Physiology and Biochemistry Unit, Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria

    Abiodun A. Onilude

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  1. Olusegun A. Olaoye
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  2. Abiodun A. Onilude
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  3. Oyeyemi A. Idowu
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Correspondence to Olusegun A. Olaoye.

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Olaoye, O.A., Onilude, A.A. & Idowu, O.A. Microbiological Profile of Goat Meat Inoculated with Lactic Acid Bacteria Cultures and Stored at 30°C for 7 days. Food Bioprocess Technol 4, 312–319 (2011). https://doi.org/10.1007/s11947-010-0343-3

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