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Enhanced Human Lysozyme Production by Kluyveromyces lactis

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Abstract

An attempt to enhance recombinant human lysozyme production by Kluyveromyces lactis K7 was performed in this study. In this study, the production of recombinant human lysozyme was investigated using shake flasks and bioreactor under different cultivation conditions. It was demonstrated that 25 °C could enhance human lysozyme production when compared with other temperatures tested. This study also demonstrated that higher biomass did not necessarily produce higher human lysozyme, and it was clear that human lysozyme production was enhanced under unfavorable conditions such as low acidity and oxygen limitation. Cultivation condition with no pH control demonstrated better production than with pH controlled near neutrality. Oxygen limitation also resulted in higher recombinant human lysozyme production. Overall human lysozyme was increased from 64.1 U/ml in flask to 123.6 U/ml in fed-batch fermentation using 4.5% (w/v) glucose initially and fed with concentrated lactose to achieve 9% lactose concentration.

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Acknowledgements

The funding for this project was provided by Pennsylvania Agricultural Experimental Station. Authors would like to have special thanks to Ms. Lisa Wilken of Texas A&M University and Dr. Mike Zhang of Virginia Tech on lysozyme analysis.

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Authors and Affiliations

  1. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA

    Eric Lu Huang & Ali Demirci

  2. The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA

    Ali Demirci

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  1. Eric Lu Huang
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  2. Ali Demirci
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Correspondence to Ali Demirci.

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Huang, E.L., Demirci, A. Enhanced Human Lysozyme Production by Kluyveromyces lactis . Food Bioprocess Technol 2, 222–228 (2009). https://doi.org/10.1007/s11947-008-0062-1

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  • DOI: https://doi.org/10.1007/s11947-008-0062-1

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