Abstract
Recent studies have shown that not only plasma B-type natriuretic peptide (BNP)-32, but also plasma proBNP-108 is increased in heart failure (HF), and that the current BNP-32 assay kit crossreacts with proBNP-108. It also was shown that both BNP-32 and proBNP-108 were higher in HF than in normal. The proBNP-108/total BNP (BNP-32 + proBNP-108) ratio was widely distributed and patients with HF with ventricular overload had higher proBNP-108/total BNP ratio than HF patients with atrial overload. Consistent with this finding, proBNP-108 was the major molecular form in ventricular tissue, and BNP-32 was the major molecular form in atrial tissue. In addition, proBNP-108 was the major molecular form of BNP in pericardial fluid. The proBNP-108/total BNP ratio increased with deterioration of HF and decreased with improvement of HF. Thus, not only BNP-32, but also proBNP-108 is increased in HF and the proBNP-108/total BNP ratio also rises in association with pathophysiological conditions such as ventricular overload. A new hypothesis that O-glycosylation at Thr71 in a region close to the cleavage site impairs proBNP-108 processing was proposed. In the future, the precise mechanism of increased proBNP-108 in HF should be elucidated.
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Acknowledgments
We thank Dr. Masashi Ikeda for helpful advice. We thank Mr. Kazumi Akimoto, Ms. Masako Matsubara, Ms. Keiko Ishikawa, Ms. Masako Minato, Ms. Kyoko Tabei, and Ms. Machiko Sakata for technical assistance.
This work was supported in part by Scientific Research Grants-in-Aid (18590787 and 20590837) from the Ministry of Education, Culture, Sports, Science and Technology of Japan; by the Fund from the Suzuken Memorial Foundation; by the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan; and by the Intramural Research Fund of National Cerebral and Cardiovascular Center of Japan.
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Nishikimi, T., Minamino, N. & Nakao, K. Diverse Molecular Forms of Plasma B-Type Natriuretic Peptide in Heart Failure. Curr Heart Fail Rep 8, 140–146 (2011). https://doi.org/10.1007/s11897-011-0051-y
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DOI: https://doi.org/10.1007/s11897-011-0051-y