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Phytosterol of Potamogeton maackianus and its change under the stress of Microcystis aeruginosa

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Wuhan University Journal of Natural Sciences

Abstract

The phytosterol in Potamogeton maackianus was identified and quantified. From the hexane extracts analysis of unsaponifiable fraction of P. maackianus by gas chromatography-mass spectrometry(GC-MS), campesterol, stigmasterol and β-sitosterol were the main phytosterols in P. maackianus with the contents of 0.368, 1.17 and 0.824 mg·g−1, respectively. After 7 days’ exposure under M. aeruginosa, the contents of campesterol, stigmasterol and β-sitosterol in P.maackianus increased by 23.1%, 40.4% and 40.8%, respectively. Both the contents of 24-ethyl sterols (sitosterol and stigmasterol) and 24-methyl sterol (campesterol) in macrophytes increased, but the percentage of total sterol composition did not change significantly. This result indicated that the content of 4-demethyl sterols and the second alkylation of the phytosterol side-chain at C-24 in P. maackianus were affected by M. aeruginosa.

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Authors and Affiliations

  1. Institute of Hydrobiology, Chinese Academy of Sciences / State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan, 430072, Hubei, China

    Shenghua Zhang, Zhenbin Wu, Shuiping Cheng, Wei Liang & Xiaohui Wu

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Shenghua Zhang

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  1. Shenghua Zhang
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  2. Zhenbin Wu
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  3. Shuiping Cheng
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  4. Wei Liang
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  5. Xiaohui Wu
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Correspondence to Zhenbin Wu.

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Foundation item: Supported by the National Science Fund for Distinguished Young Scholars (39925007) and the National High Technology Research and Development Program of China (863 Program) (2002AA601021)

Biography: ZHANG Shenghua(1979–), female, Ph.D. candidate, research direction: environmental biology.

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Zhang, S., Wu, Z., Cheng, S. et al. Phytosterol of Potamogeton maackianus and its change under the stress of Microcystis aeruginosa . Wuhan Univ. J. Nat. Sci. 13, 113–117 (2008). https://doi.org/10.1007/s11859-008-0122-4

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  • DOI: https://doi.org/10.1007/s11859-008-0122-4

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