Abstract
The cytotoxic effect of cadmium is studied by detecing intracellular nicotinamide adenine dinucleotidea(NADH) autofluorescence in this work. NADH autofluorescence in processes of cadmium-induced apoptosis, necrosis and reversible injury are recorded timely. The relativity between time course of NADH autofluorescence and cadmium toxicity is established. The cell toxicity effect of Cadmium on yeast cells is studied by detecting the time courses of intracellular reduced NADH autofluorescence in this work. The relativity between time courses of NADH autofluorescence and Cadmium toxicity is established.
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References
Wang J, Cai R X, Xu J, et al. Study on the Effect of Thiamine on the Metabolism of Yeast by Intrinsic Fluorescence [J]. Luminescence, 2005, 20: 216–219.
Lin S J, Guarente L. Nicotinamide Adenine Dinucleotide, a Metabolic Regulator of Transcription, Longevity and Disease [J]. Curr Opin Cell Biol, 2003, 15: 241–246.
Klaidman L K, Mukherjee S K. Oxidative Changes in Brain Pyridine Nucleotides and Neuroprotection Using Nicotinamide [J]. Biochimica et Biophysica Acta, 2001, 1525: 136–148.
Goering P L, Waalkes M P, Klaassen C D. Toxicology of Cadmium. Toxicology of Metals: Biochemical Aspects [J]. Handbook of Experimental Pharmacology, 1995, 15: 189–213.
Faverney C R D, Orsini N, Sousa G D, et al. Cadmium-Induced Apoptosis Through the Mitochondrial Pathway in Rainbow Trout Hepatocytes: Involvement of Oxidative stress [J]. Aquatic Toxicology, 2004, 69: 247–258.
Priault M, Camougrand N, Kinnally K W. Yeast as a Tool to Study Bax/Mitochondrial Interactions in Cell Death[J]. FEMS Yeast Research, 2003, 4: 15–27.
Kalvelyte A, Imbrasaite A, Bukauskiene A, et al. Connexins and Apoptotic Transformation [J]. Biochem Pharmacol, 2003, 66: 1661–1672.
Chen Y H, Liu Z H, Cai R X, et al. Determination of Intracellular NAD (P)H Level [J]. Progress in Chemistry, 2004, 16: 962–968.
Zhang Z Q, Yu J, Stanton R C. A Method for Determination of Pyridine Nucleotides Using a Single Extract. Analytical Biochemistry, 2000, 285: 163–167.
Chowdhury S K R, Drahota Z D, Floryk P, et al. Activities of Mitochondrial Oxidative Phosphorylation Enzymes in Cultured Amniocytes [J]. Clin Chim Acta, 2000, 298: 157–173.
Carson D A, Seto S, Wasson D B, et al. DNA Strand Breaks, NAD Metabolism, and Programmed Cell Death [J]. Exp Cell Res, 1986, 164: 273–281.
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Foundation item: Supported by the National Natural Science Foundation of China (20275027)
Biography: LIANG Ju(1980–), female, Associate professor, research direction: pharmaceutical analysis.
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Liang, J., Cai, R., Wu, W. et al. Cell toxicity effect of cadmium on yeast cells by detecting NADH autofluorescence. Wuhan Univ. J. of Nat. Sci. 12, 333–336 (2007). https://doi.org/10.1007/s11859-006-0033-1
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DOI: https://doi.org/10.1007/s11859-006-0033-1