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Quantitative detection of methylated SOCS-1 in schizophrenia and bipolar disorder considering SOCS-1 -1478CA/del polymorphism and clinical parameters

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Abstract

Background

We aimed to investigate the quantitative detection of methylated suppressor of cytokine signaling-1 (SOCS-1) in schizophrenia (SCZ) and bipolar disorder (BD), considering SOCS-1 -1478CA/del polymorphism and clinical parameters.

Methods

Our research is a case–control study in which 114 patients with SCZ, 86 patients with BD, and 80 volunteers as a healthy group participated. Bisulfite-converted DNA samples were analyzed using the real-time quantitative methylation-specific PCR (qMS-PCR) method to measure the methylation level of the SOCS-1 gene. In addition, SOCS-1 -1478CA/del gene polymorphism was analyzed with the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP).

Results

When the SOCS-1 promoter methylation levels of SCZ and BD patients were compared with the control group, the methylation levels of SCZ and BD were significantly lower than the control group. An earlier age of illness onset was significantly related to the SOCS-1 promoter hypermethylation in DNA samples of SCZ patients. Again, SOCS-1 promoter hypermethylation was significantly associated with the higher Young Mania Rating Scale (YMRS) score in BD patients. While the SOCS-1 CA/CA genotype frequency was significantly higher in the control group than in the BD group, the del/del genotype was significantly related to a higher frequency of rapid cycling and a lower frequency of family history in the BD patient group.

Conclusion

In summary, the methylated SOCS-1 quantity in DNA samples of SCZ and BD patients were significantly lower than in control samples. Whereas the SOCS-1 -1478CA/del polymorphism was not related to SCZ, it may be associated with the BD.

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Acknowledgements

We want to thank all patients and healthy controls for their willingness to participate in the present study.

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Authors and Affiliations

  1. Department of Psychiatry, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey

    Hasan Mervan Aytac

  2. Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

    Sacide Pehlivan & Yasemin Oyaci

  3. Department of Hematology, Gaziantep University, Faculty of Medicine, Gaziantep, Turkey

    Mustafa Pehlivan

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  1. Hasan Mervan Aytac
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  2. Sacide Pehlivan
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  4. Yasemin Oyaci
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Contributions

HMA and SP are responsible for developing research goals and aims. HMA, SP, and YO conceived and designed the study. SP and YO are the responsible requirements for laboratory samples. HMA and MP acquired, analyzed, and interpreted all data. HMA prepared the manuscript. SP and MP supervised.

Corresponding author

Correspondence to Hasan Mervan Aytac.

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The authors express that all procedures contributing to this work comply with the suitable national and institutional committees’ ethical standards on human experimentation and the Helsinki Declaration of 1975, as revised in 2013 [22].

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The authors declare no competing interests.

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Aytac, H.M., Pehlivan, S., Pehlivan, M. et al. Quantitative detection of methylated SOCS-1 in schizophrenia and bipolar disorder considering SOCS-1 -1478CA/del polymorphism and clinical parameters. Ir J Med Sci 192, 775–783 (2023). https://doi.org/10.1007/s11845-022-03030-w

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  • DOI: https://doi.org/10.1007/s11845-022-03030-w

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