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Circulating miR-342-5p serves as a diagnostic biomarker in patients with carotid artery stenosis and predicts the occurrence of the cerebral ischemic event

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Abstract

Background

Carotid artery stenosis (CAS) is an important risk factor for cerebral ischemia events (CIE). Previous studies have shown that microRNAs (miRNAs) are involved in the occurrence and development of CAS.

Aims

The purpose of this study was to reveal the clinical diagnostic value of miR-342-5p for asymptomatic CAS (ACAS) and to evaluate its predictive value for the occurrence of CIE in patients.

Methods

A total of 92 ACAS patients and 86 healthy controls were enrolled as subjects. The expression level of serum miR-342-5p was detected by qRT-PCR. The receiver operating characteristic (ROC) curve was used to detect the diagnostic value of miR-342-5p in ACAS. Kaplan–Meier survival and Cox regression analysis assessed the predictive value of miR-342-5p for the occurrence of CIE in ACAS patients.

Results

The level of serum miR-342-5p in ACAS patients was significantly higher than that in healthy controls (P < 0.05). ROC curve showed the high diagnostic value of serum miR-342-5p, which could distinguish ACAS patients from healthy controls. Multivariate Cox regression analysis confirmed that miR-342-5p was an independent predictor (HR = 5.512, 95%CI = 1.370–22.176, P = 0.016). What is more, Kaplan–Meier analysis confirmed that patients with high miR-342-5p expression develop more CIE (log-rank, P = 0.020).

Conclusions

miR-342-5p was significantly overexpressed in ACAS. And the upregulation of serum miR-342-5p is a valuable diagnostic biomarker and can predict the occurrence of CIE.

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Weifang Medical University, Shandong, 261031, China

    Aihua Zhou, Ping Yu & Liying Lang

  2. Department of Urology Surgery, Affiliated Hospital of Weifang Medical University, Shandong, 261031, China

    Ying Li

  3. Department of Respiratory Medicine, Affiliated Hospital of Weifang Medical University, Shandong, 261031, China

    Ping Wang

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  1. Aihua Zhou
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  2. Ying Li
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  3. Ping Wang
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  4. Ping Yu
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  5. Liying Lang
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Correspondence to Aihua Zhou.

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Ethics approval

The study protocol was approved and authorized by the Ethics Committee of Affiliated Hospital of Weifang Medical University. The procedures of the patients involved in the study followed the guidelines of the Declaration of Helsinki.

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The patients signed and provided informed consent.

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The authors declare no competing interests.

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Cite this article

Zhou, A., Li, Y., Wang, P. et al. Circulating miR-342-5p serves as a diagnostic biomarker in patients with carotid artery stenosis and predicts the occurrence of the cerebral ischemic event. Ir J Med Sci 191, 713–718 (2022). https://doi.org/10.1007/s11845-021-02623-1

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  • DOI: https://doi.org/10.1007/s11845-021-02623-1

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