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Bioevaluation of Subabul (Leucaena leucocephala) proteinase inhibitors on Helicoverpa armigera

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Abstract

Helicoverpa armigera, a highly polyphagous pest, has a broad host spectrum, causes significant levels of yield loss in many agriculturally important crops. Serine primarily responsible for most of the proteolytic activity in the larval gut of lepidopteron insects. Neonate larvae were reared on artificial diet and chickpea seeds smeared with Subabul Trypsin Inhibitor. Larvae fed with artificial diet showed reduction in larval weight up to 21% (HSTI) and 43% (LSTI). However, larvae fed on seeds showed significant reduction in weight, 52.4% (HSTI) and 60.3% (LSTI), suggesting that the diet also plays a vital role on the effectiveness of the inhibitors on larval growth and development. HSTI and LSTI inhibited the gut proteinases from larvae fed on artificial diet significantly (41.40% and 64.36%) compared to the gut proteinases (27.80% and 38.90%) from larvae fed on chickpea seeds. Seeds smeared with 10,000 TIU resulted in complete mortality of larvae while there was no mortality observed in artificial diet. The results reveal that LSTI is a stronger inhibitor of insect gut proteinases and for larvae fed with poor nutrition in the natural ecosystems, low level expression of inhibitor would be enough to affect the growth and development.

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References

  • Broadway RM, Duffey SS (1986a) The effect of dietary protein on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua. J Insect Physiol 32:673–680

    Article  CAS  Google Scholar 

  • Broadway RM, Duffey SS (1986b) Plant proteinase inhibitors: mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua. J Insect Physiol 32:827–833

    Article  CAS  Google Scholar 

  • Chougule NP, Hivrale VK, Chhabda PJ, Giri AP, Kachole MS (2003) Differential inhibition of Helicoverpa armigera gut proteinases by proteinase by proteinase inhibitors of pigeonpea, Cajanus cajan, and its wild relatives. Phytochemistry 64:681–687

    Article  PubMed  CAS  Google Scholar 

  • Filho JX, Moreira RDA (1978) Visualization of proteinase inhibitors in SDS-polyacrylamide gels. Anal Biochem 84:296–299

    Article  PubMed  CAS  Google Scholar 

  • Fitt GP (1989) The ecology of Heliothis in relation to agroecosystems. Annu Rev Entomol 34:17–52

    Article  Google Scholar 

  • Franco OL, dos Santos RC, Batista JA, Mendes AC, de Araujo MA, Monnerat RG, Grossi-de-Sa MF, de Freitas SM (2003) Effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of Anthonomus grandis. Phytochemistry 63:343–349

    Article  PubMed  CAS  Google Scholar 

  • Gatehouse AMR, Gatehouse JA (1998) Iddentifying proteins with insecticidal activity and use of encoding genes to produce insect resistant transgenic crops. Pestic Sci 52:165–175

    Article  CAS  Google Scholar 

  • Giri AP, Harsulkar AM, Ku MS, Gupta VS, Deshpande VV, Ranjekar PK, Franceschi VR (2003) Identification of potent inhibitors of Helicoverpa armigera gut proteinases from winged bean seeds. Phytochemistry 63:523–532

    Article  PubMed  CAS  Google Scholar 

  • Gomes CE, Barbosa AE, Macedo LL, Pitanga JC, Moura FT, Oliveira AS, Moura RM, Queiroz AF, Macedo FP, Andrade LB, Vidal MS And Sales MP, 2005, Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus cowpea weevil and Ceratitis capitata fruit fly. Plant Physiol Biochem 4312:1095–1102

    Article  Google Scholar 

  • Heckel DG, Gahan LJ, Gould F, Daly JC, Trowell S (1997) Genetics of Heliothis and Helicoverpa Resistance to Chemical insecticide and to Bacillus thuringiensis. Pestic Sci 51:251–258

    Article  CAS  Google Scholar 

  • Haq SK, Atif SM, Khan RH (2004) Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection. Arch Biochem Biophys 431:145–159

    Article  PubMed  CAS  Google Scholar 

  • Jouanin L, Bonade-Bottina M, Girard C, Morrot G, Giband M (1998) Transgenic plants for insect resistances. Plant Sci 131:1–11

    Article  CAS  Google Scholar 

  • Kakade ML, Simons N, Liener IE (1969) An evaluation of natural and synthetic substrates for the antitryptic activity of soybean samples. Cereal Chem 46:518–522

    CAS  Google Scholar 

  • Koiwa H, Bressan RA, Hasegawa PM (1997) Regulation of protease inhibitors and plant defense. Trends Plant Sci 2:379–384

    Article  Google Scholar 

  • Lawrence PK And Koundal KR, 2002, Plant protease inhibitors in control of phytophagous insects. Electron J Biotechnol 5:93–109

    Google Scholar 

  • Nandeesha, P (2000) Biochemical Characterization of serine Proteinase inhibitor from subabul seeds. MSc (Biotechnolgy) thesis, University of Agricultural Sciences, Bangalore

  • Nandeesha P, Prasad DT (2001) Characterization of serine proteinase inhibitor from subabul (Leucaena leucocephala. L) seeds. J Plant Biochem Biotechnol 10:75–78

    CAS  Google Scholar 

  • Oliva ML, Souza-pinto JC, Batista IF, Araujo MS, Silveria VF , Aureswald EA, Mentele R, Eckerskorn C, Sampaio MU, Sampaio CA (2000) Leucaena leucocephala serine proteinase inhibitor: primary structure and action on blood coagulation kinin release and rat paw edema. Biochim Biophys Acta 1477:64–74

    PubMed  CAS  Google Scholar 

  • Patankar AG, Giri AP, Harsulkar AM, Sainani MN, Deshpande VV, Ranjekar PK, Gupta VS. 2001. Complexity in specificities and expression of Helicoverpa armigera gut proteinases explains polyphagous nature of the insect pest. Insect Biochem Mol Biol 31:453–64

    Article  PubMed  CAS  Google Scholar 

  • Patrícia P, Maria CF, Jose Roberto PP, Marcio CS (2003) Coupling diet quality and Bowman-Birk and Kunitz-type soybean proteinase inhibitor effectiveness to Diatraea saccharalis development and mortality. Entomol Exp Applic 109:217

    Article  Google Scholar 

  • Pearce G, Sy L, Russel C, Ryan CA, Hass M (1982) Isolation and characterization from potato tubers of two polypeptide inhibitors of serine proteases. Arch Biochem Biophys 213:456–462

    Article  PubMed  CAS  Google Scholar 

  • Rodrigues ML, Machado Freire MG, Cabrini EC, Toyama MH, Novello JC, Marangoni S (2003) A trypsin inhibitor from Peltophorum dubium seeds active against pest proteases and its effect on the survival of Anagasta kuehniella (Lepidoptera: Pyralidae). Biochim Biophys Acta 1621:170–182

    Google Scholar 

  • Ryan CA (1981) Proteinase inhibitors, a comprehensive treatise, In: The Biochemistry of Plants 6:351–370

  • Sattar R, Ali SA, Kamal M, Khan AA, Abbasi A (2004) Molecular mechanism of enzyme inhibition: prediction of the three-dimensional structure of the dimeric trypsin inhibitor from Leucaena leucocephala by homology modeling. Biochem Biophys Res Commun 314:755–765

    Article  PubMed  CAS  Google Scholar 

  • Schuler TH, Poppy GM, Kerry BR, Denholm I (1998) Insect resistant transgenic plants. Trends Biotechnol 16:168–175

    Article  CAS  Google Scholar 

  • Ussuf KK, Laxmi NH, Mitra R (2001) Proteinase inhibitors: plant derived genes of insecticidal protein for developing insect resistant transgenic plants. Curr Sci 80:847–850

    CAS  Google Scholar 

  • Vaijayanti AT, Nanasaheb PC, Ashok PG, Anirudha RD, Mohini NS, Vidya SG, 2005, In vivo and in vitro effect of Capsicum annum proteinase inhibitors on Helicoverpa armigera gut proteinases. Biochim Biophys Acta 1722:156–167

    Google Scholar 

  • Veerareddy CG, Battacharya AK (1990) Development of semi synthetic diets for the rearing of Helicoverpa armigera (Hub.). J Insect Sci 3:23–33

    Google Scholar 

  • Volpicella M, Ceci RL, Cordewener J, America T, Gallerani R, Bode W, Jongsma MA, Beekwilder J (2003) Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors. Eur J Biochem 270:10–19

    Article  PubMed  CAS  Google Scholar 

  • Zhang JH, Wang CZ, Qin JD, Guo SD (2004) Feeding behaviour of Helicoverpa armigera larvae on insect-resistant transgenic cotton and non-transgenic cotton. J Appl Entomol 128:218–225

    Article  Google Scholar 

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Acknowledgements

We thank Dr. N. S. Bhat for his useful suggestions and Biopest Management unit, Ceekay Seeds (India) for providing the insects for the experiments.

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  1. Biotechnology Department, University of Agricultural Sciences, GKVK, Bangalore, 560065, India

    P. Bhavani, Chumki Bhattacherjee & D. Theertha Prasad

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  1. P. Bhavani
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  2. Chumki Bhattacherjee
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  3. D. Theertha Prasad
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Correspondence to D. Theertha Prasad.

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Handling editor: Chen-Zhu Wang

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Bhavani, P., Bhattacherjee, C. & Theertha Prasad, D. Bioevaluation of Subabul (Leucaena leucocephala) proteinase inhibitors on Helicoverpa armigera . Arthropod-Plant Interactions 1, 255–261 (2007). https://doi.org/10.1007/s11829-007-9020-5

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