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Ionophoric activity of truncated gramicidin A analogue in phospholipid bilayers and mitochondrial and erythrocyte membranes

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Ionophoric activities of an N-terminus truncated gramicidin A (gA) analogue (mini-gramicidin) and its covalent dimer were studied in planar bilayer phospholipid membranes (BLM) using macroscopic current measurements (at high concentrations of the peptides) and single-channel recordings. As with gA-induced currents, mini-gramicidin-stimulated macroscopic currents through BLM underwent sensitized photoinactivation, i.e. were suppressed after irradiation with visible light in the presence of a photosensitizer generating singlet oxygen. The sensitivity of the tested compounds to photoinactivation descended in the following order: minigramicidin dimer > mini-gramicidin monomer > gA. The data from single-channel measurements and kinetic analysis of flash-induced photoinactivation obtained at different levels of macroscopic currents suggest that mini-gramicidin and its covalent dimer induce a variety of conducting states; their ratio depends on membrane thickness. Analysis of natural (mitochondrial and erythrocyte) membranes established that ionophoric activities of mini-gramicidin and its covalent dimer depend essentially on the membrane type.

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Abbreviations

BLM:

bilayer lipid membrane

AlPcS3 :

trisulfonated aluminum phthalocyanine

DPhPC:

diphytanoylphosphaditylcholine

POPC:

palmitoyloleoylphosphaditylcholine

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Vorob’evy gory, Moscow, 119991, Russia

    E. A. Dutseva, E. A. Kotova & Yu. N. Antonenko

  2. School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Vorob’evy gory, Moscow, 119991, Russia

    E. A. Dutseva

Authors
  1. E. A. Dutseva
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  2. E. A. Kotova
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  3. Yu. N. Antonenko
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Additional information

Original Russian Text © E.A. Dutseva, E.A. Kotova, Yu.N. Antonenko, 2008, published in Biologicheskie Membrany, 2008, Vol. 25, No. 1, pp. 58–65.

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Dutseva, E.A., Kotova, E.A. & Antonenko, Y.N. Ionophoric activity of truncated gramicidin A analogue in phospholipid bilayers and mitochondrial and erythrocyte membranes. Biochem. Moscow Suppl. Ser. A 2, 55–61 (2008). https://doi.org/10.1007/s11827-008-1009-4

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  • DOI: https://doi.org/10.1007/s11827-008-1009-4

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