Abstract
The increasing cultivation and use of genetically modified (GM) crops has increased the importance of environmental monitoring for safety management. Brassica napus is the fourth most commonly cultivated GM crop globally, and it can hybridize with Brassica rapa or Brassica juncea by serving as a pollen donor or recipient for these species. When a hybrid of B. rapa or B. juncea with GM B. napus is detected in an outdoor environment, the safety management goals could determine whether B. napus is a pollen donor or recipient. In this study, two PCR (polymerase chain reaction) markers were developed to identify the paternal and maternal involvement of B. napus in hybrids with B. rapa or B. juncea based on the chloroplast genome data of B. napus. Further, Marker primer 07 differentiated B. napus from the other Brassica species, and Marker primer 20 differentiated B. napus from B. rapa and B. juncea. Applying the chloroplast and nuclear markers of the C and B genomes to the hybrids B. napus × B. rapa, B. rapa × B. napus, and B. juncea × B. napus using the PCR method showed that B. napus, B. rapa, B. juncea, and all hybrids could be distinguished. Thus, the effectiveness of these markers in identifying hybrids of GM B. napus in the natural environment has been demonstrated for environmental monitoring.
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Acknowledgements
This work was supported by the Technology Innovation Program (20014752, Development of industrial LMO monitoring technology in production and utilization facilities) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
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The funding has been received from Ministry of Trade, Industry and Energy with Grant no. 20014752.
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Lee, B., Sohn, SI. Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids. Plant Biotechnol Rep 17, 489–497 (2023). https://doi.org/10.1007/s11816-023-00848-3
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DOI: https://doi.org/10.1007/s11816-023-00848-3