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Overexpression of a Brassica rapa MADS-box gene, BrAGL20, induces early flowering time phenotypes in Brassica napus

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Abstract

BrAGL20 (SOC1) containing MADS box, a floral integrator gene, was introduced into Brassica napus cv. “Youngsan” by Agrobacterium-mediated transformation. Constitutively overexpressed BrAGL20 under the CaMV 35S promoter induced early flowering time compared to the wild-type. These phenotypes were stably inherited through generations T2 and T3, regardless of planting season. The expression of the floral meristem identity genes LFY and AP1 seemed to appear rapidly in the shoot apex region of transgenic plants showing the early flowering time phenotype. These results suggest that overexpression of BrAGL20 can significantly affect the flowering time of B. napus, and regulation of floral integrator gene expression could be applied for adaptation of crops to local environments and climate changes.

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Acknowledgments

This work was carried out with the support of “Research Program for Agricultural Biotechnology (PJ907049)”, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea. J.K. Hong was supported by a 2012 Post-Doctoral Fellowship Program of National Academy of Agricultural Science, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea

    Joon Ki Hong, Soo-Jin Kwon, Jung Sun Kim, Jin A. Kim, Soo In Lee & Yeon-Hee Lee

  2. National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 441-707, Korea

    Soo-Yun Kim

  3. Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Suwon, 441-707, Korea

    Kwang-Soo Kim

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  1. Joon Ki Hong
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  2. Soo-Yun Kim
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Correspondence to Yeon-Hee Lee.

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Hong, J.K., Kim, SY., Kim, KS. et al. Overexpression of a Brassica rapa MADS-box gene, BrAGL20, induces early flowering time phenotypes in Brassica napus . Plant Biotechnol Rep 7, 231–237 (2013). https://doi.org/10.1007/s11816-012-0254-z

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