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Effect of localized reduction of gibberellins in different tobacco organs on drought stress tolerance and recovery

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Abstract

Drought resistance is increased in plants by the absence of the hormone gibberellic acid (GA) or by a lack of GA sensitivity. We studied the effects of tissue-specific reduction in GA levels on drought tolerance, on recovery from drought stress, and on primary and secondary growth using transgenic tobacco plants expressing the GA-inactivating gene PtGA2ox 1 (GA 2-oxidase) specifically in leaves, stems, or roots. Localized reduction of bioactive GA1 levels was achieved by tissue-specific expression of the PtGA2ox 1 gene in leaves using the rbcs promoter (LD plants), in roots using the TobRB7 promoter (RD plants), and in stems using the LMX5 promoter (SD plants). In response to drought stress, all transgenic tobacco plants exhibited reduced primary and secondary growth and increased drought tolerance with a corresponding reduction in malondialdehyde levels, higher relative water content, increased proline and sugar content, and elevated peroxidase, superoxide dismutase, and catalase activities relative to wild-type plants. The highest level of drought tolerance and the most rapid recovery from stress was achieved by localized reduction of GA1 in the roots of the RD transgenic plants. In addition, although the total bioactive GA1 content in RD and LD plants was essentially identical, the heights of LD plants were significantly greater and drought tolerance was significantly less than in RD plants. It is possible that the site of gibberellin-related gene expression plays an important role in the balance between growth and drought tolerance.

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Acknowledgments

We thank Dr. Shihui Niu (Beijing Forestry University) for providing cultured transgenic tobacco seedlings. This work was supported by a grant from the National Natural Science Foundation of China (31172255), the National High Technology Research and Development Program of China (2013AA102607), and the Introduction of International Advanced Agricultural Technology Program of China (2011-4-50).

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  1. Beijing Forestry University, Beijing, 100083, China

    Tianxiu Zhong, Lijuan Zhang, Shanshan Sun, Huiming Zeng & Liebao Han

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  1. Tianxiu Zhong
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  2. Lijuan Zhang
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Correspondence to Liebao Han.

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Zhong, T., Zhang, L., Sun, S. et al. Effect of localized reduction of gibberellins in different tobacco organs on drought stress tolerance and recovery. Plant Biotechnol Rep 8, 399–408 (2014). https://doi.org/10.1007/s11816-014-0330-7

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