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High-frequency shoot multiplication in Artemisia vulgaris L. using thidiazuron

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Abstract

An in vitro propagation system for Artemisia vulgaris L., a traditional medicinal plant, has been developed. The best organogenic response, including adventitious shoot number and elongation, was obtained when hypocotyl segments were cultured onto MS medium supplemented with 4.54 μM TDZ (N-phenyl-N′-(1,2,3-thidiazol-yl) urea). Up to 28 shoots formed per explant for an optimal duration of exposure of 48 days. Regenerated shoots formed roots when subcultured onto a medium containing 8.56 μM IAA (indole-3-acetic acid). Healthy plantlets were transferred to a garden soil:farmyard soil:sand (2:1:1) mixture for acclimatization, which was successful, and subsequent maturity was achieved under greenhouse conditions over a six-month period. The survival rate of the plantlets varied under acclimatization. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. vulgaris. This optimized protocol has been successfully employed for genetic transformation studies in A. vulgaris, which are currently underway in our laboratory.

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  1. Medicinal Plant Biotechnology Division, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    G. Sujatha & B. D. Ranjitha Kumari

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  1. G. Sujatha
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  2. B. D. Ranjitha Kumari
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Correspondence to B. D. Ranjitha Kumari.

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Sujatha, G., Ranjitha Kumari, B.D. High-frequency shoot multiplication in Artemisia vulgaris L. using thidiazuron. Plant Biotechnol Rep 1, 149–154 (2007). https://doi.org/10.1007/s11816-007-0028-1

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