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Synthesis of fluorescent dye-embedded silica nanoparticles for vitamin D3 detection using sandwich-like assay

  • Materials (Organic, Inorganic, Electronic, Thin Films)
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Abstract

This study devised and examined a sandwich-like assay for detecting vitamin D3 dissolved in acetonitrile using fluorescent dye-embedded silica nanoparticles (AF647@SiNPs). First, AF647@SiNPs were synthesized via the modified Stöber method to embed the fluorescent dye molecules in the silicate network. Then, vitamin D3 (sample) was immobilized using an azide linker on the magnetic nanoparticles (MNPs). The immobilized vitamin D3 reacted with the antibody immobilized on the surface of AF647@SiNPs (Anti-VD3@AF647@SiNPs). The vitamin D3 concentration was calculated back using the fluorescence of the remaining unreacted Anti-VD3@AF647@SiNPs. Finally, we examined how applying conditions of AF647@SiNPs influenced the measurement reliability: (1) the initial loading of AF647@SiNPs; (2) the antibody-labeling degree of AF647@SiNPs.

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References

  1. F. H. Cincotto, T. C. Canevari and S. A. S. Machado, Electroanalysis, 26(12), 2783 (2014).

    Article  CAS  Google Scholar 

  2. E. Kasalova, J. Aufartova, L. K. Krcmova, D. Solichova and P. Solich, Food Chem., 171, 177 (2015).

    Article  CAS  Google Scholar 

  3. E. J. Dufek, B. Ehlert, M. C. Granger, T. M. Sandrock, S. L. Legge, M. Herrmann, A. W. Meikle and M. D. Porter, Analyst, 135(11), 2811 (2010).

    Article  CAS  Google Scholar 

  4. A. M. Wallace, S. Gibson, A. de la Hunty, C. Lamberg-Allardt and M. Ashwell, Steroids, 75(7), 477 (2010).

    Article  CAS  Google Scholar 

  5. S. Kia, M. Fazilati, H. Salavati and S. Bohlooli, RSC Adv., 6(38), 31906 (2016).

    Article  CAS  Google Scholar 

  6. X. He, P. Jiang, Y. Xue, W.-Y. Zhu, Y. Deng, M. Yan, H.-D. Li, R.-L. Dang and M.-M. Tang, Methods, 8(11), 2400 (2016).

    CAS  Google Scholar 

  7. H. Dobnig, S. Pilz, H. Scharnagl, W. Renner, U. Seelhorst, B. Wellnitz, J. Kinkeldei, B. Boehm, G. Weihrauch and W. Maerz, Arch. Intern. Med., 12, 1340 (2008).

    Article  Google Scholar 

  8. K. Galior, S. Grebe and R. Singh, Nutrients, 10(8) 953 (2018).

    Article  Google Scholar 

  9. B. W. Hollis, J. Q. Sandra, S. R. Selvaag, J. D. Lorenz and J. L. Napoli, Clin. Chem., 39, 529 (1993).

    Article  CAS  Google Scholar 

  10. D. Chauhan, R. Kumar, A. K. Panda and P. R. Solanki, J. Mat. Res. Tech., 8(6), 5490 (2019).

    Article  CAS  Google Scholar 

  11. D. Chauhan, P. K. Gupta and P. R. Solanki, Mater. Sci. Eng. C., 93, 145 (2018).

    Article  CAS  Google Scholar 

  12. M. K. Koivula, N. Matinlassi, P. Laitinen and J. Risteli, Clin. Lab., 59(3–4), 397 (2013).

    CAS  Google Scholar 

  13. S. Lee, J.-H. Kim., S.-A. Kim, Y.-S. Sun, A. Lee, S.-J. Park, Y.-T. Kim, K.-R. Lee and Y.-J. Kim, Ann. Clin. Lab. Sci., 46, 645 (2016).

    CAS  Google Scholar 

  14. M. J. Bogusz, E. Al Enazi, M. Tahtamoni, J. A. Jawaad and M. A. Tufail, Clin. Biochem., 44(16), 1329 (2011).

    Article  CAS  Google Scholar 

  15. R. D. Coldwell, D. J. Trafford, M. J. Varley, D. N. Kirk and H. L. J. Makin, Clinica Chimica Acta, 180, 157 (1989).

    Article  CAS  Google Scholar 

  16. G. D. Carter and J. C. Jones, Ann. Clin. Biochem., 46, 79 (2009).

    Article  CAS  Google Scholar 

  17. A. P. D. Leenheer and A. A. Cruyl, Anal. Biochem., 91, 293 (1978).

    Article  Google Scholar 

  18. P. B. Luppa, C. Muller, A. Schlichtiger and H. Schlebusch, Trends. Anal. Chem., 30(6), 887 (2011).

    Article  CAS  Google Scholar 

  19. R. F. Louie, Z. Tang, D. G. Shelby and G. J. Kost, Lab. Med., 31, 402 (2000).

    Article  Google Scholar 

  20. E. Herz, H. Ow, D. Bonner, A. Burns and U. Wiesner, J. Mater. Chem., 19(35), 6341 (2009).

    Article  CAS  Google Scholar 

  21. H. Ow, D. R. Larson, M. Srivastava, B. A. Baird, W. W. Webb and U. Wiesner, Nano Lett., 5(1), 113 (2005).

    Article  CAS  Google Scholar 

  22. F. Hao, J. Zheng, D. Ouyang, W. Xiong, P. Liu and H. Luo, Korean J. Chem. Eng., 38(4), 736 (2021).

    Article  CAS  Google Scholar 

  23. S. B. Jo, H. J. Chae, T. Y. Kim, J.-I. Baek, D. Ragupathy, S. C. Lee and J. C. Kim, Korean J. Chem. Eng., 37(12), 2317 (2020).

    Article  CAS  Google Scholar 

  24. S. Saleh, A. Younis, R. Ali and E. Elkady, Korean J. Chem. Eng., 36(4), 529 (2019).

    Article  CAS  Google Scholar 

  25. S. Liang, C. L. John, S. Xu, J. Chen, Y. Jin, Q. Yuan, W. Tan and J. X. Zhao, in Advanced fluorescence reporters in chemistry and biology II, A. Demchenko Eds., Springer, Berlin-Heidelberg (2010).

  26. D. Tarn, C. E. Ashley, M. Xue, E. C. Carnes, J. I. Zink and C. J. Brinker, Acc. Chem. Res., 46(3), 792 (2013).

    Article  CAS  Google Scholar 

  27. H. Mader, X. Li, S. Saleh, M. Link, P. Kele and O. S. Wolfbeis, Ann. N. Y. Acad. Sci., 1130, 218 (2008).

    Article  CAS  Google Scholar 

  28. W. Yang, C. G. Zhang, H. Y. Qu, H. H. Yang and J. G. Xu, Analytica Chimica Acta, 503(2), 163 (2004).

    Article  CAS  Google Scholar 

  29. T. González-García, S. Fernández, E. Lubian, F. Mancin and M. Ferrero, RSC Adv., 6(38), 31840 (2016).

    Article  Google Scholar 

  30. J.-H. Lim, S.-W. Ha and J.-K. Lee, Bull. Kor. Chem. Soc., 33(3), 1067 (2012).

    Article  CAS  Google Scholar 

  31. D. Genovese, S. Bonacchi, R. Juris, M. Montalti, L. Prodi, E. Rampazzo and N. Zaccheroni, Angew. Chem. Int. Ed., 52(23), 5965 (2013).

    Article  CAS  Google Scholar 

  32. R. Tapec, X. J. Zhao and W. Tan, J. Nanosci. Nanotechnol., 2(3–4), 405 (2002).

    Article  CAS  Google Scholar 

  33. J. A. Funel and S. Abele, Angew. Chem. Int. Ed., 52(14), 3822 (2013).

    Article  CAS  Google Scholar 

  34. P. A. Aronov, L. M. Hall, K. Dettmer, C. B. Stephensen and B. D. Hammock, Anal. Bioanal. Chem., 391(5), 1917 (2008).

    Article  CAS  Google Scholar 

  35. G. A. Abernethy, Anal. Bioanal. Chem., 403(5), 1433 (2012).

    Article  CAS  Google Scholar 

  36. Ž. T. Rakuša, M. Pišlar, A. Kristl and R. Roška, Pharmaceutics, 13(5), 617 (2021).

    Article  Google Scholar 

Download references

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science & ICT (MSIT) (NRF-2016R1 A5A1009592).

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Authors and Affiliations

  1. Department of Chemical Engineering, Division of Chemical and Biological Engineering, Kangwon National University, Gangwon-do, Chuncheon-si, 24341, Korea

    Namhun Lee, Seongsoo Kim, Sang-Myung Lee & Dae-Won Lee

  2. Division of Mechanical & Biomedical, Mechatronics and Materials Science and Engineering, Kangwon National University, Gangwon-do, Chuncheon-si, 24341, Korea

    Kwang-Ho Lee

  3. Department of Research and Development, Cantis Inc., Ansan-si, Gyeonggi-do, 15588, Korea

    Sang-Myung Lee

Authors
  1. Namhun Lee
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  2. Seongsoo Kim
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  5. Dae-Won Lee
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Correspondence to Kwang-Ho Lee, Sang-Myung Lee or Dae-Won Lee.

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Lee, N., Kim, S., Lee, KH. et al. Synthesis of fluorescent dye-embedded silica nanoparticles for vitamin D3 detection using sandwich-like assay. Korean J. Chem. Eng. 39, 3473–3481 (2022). https://doi.org/10.1007/s11814-022-1221-1

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  • DOI: https://doi.org/10.1007/s11814-022-1221-1

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