Abstract
Prunus spp. and locally available plants (used as folkloric medicine) were screened to find a novel and natural anti-melanogenic agent. Based on p-protein promoter reporter assay (PPRA) the candidate plants were screened in the quest for p-protein inhibitor. Expression profiling of key proteins revealed the molecular mechanism of the melanin inhibition as well as TEM analysis revealed melanosome structure. The screened plant extract through PPRA showed significant down regulation of p-protein, which led to melanin inhibition. Another key melanosomal protein like tyrosinase and TRP-1 was also found to be down-regulated. However, TRP-2 was not affected. TEM analysis of treated cells also revealed that the stage IV melanosomes were lowered in number compared to control. The present study shows the plants used in this study possess good anti-melanogenic properties. However, the P. davidiana has the highest anti-melanogenic property among screened plant extracts.
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V.K. Morya, C. Ahn, S. Jeon and E.K. Kim, Mini. Rev. Med. Chem., 13, 12 (2013).
V. K. Morya, N. H. Dung, B. K. Singh, H. B. Lee and E. K. Kim, Exp. Dermatol., 23 (2014).
S. Park, V.K. Morya, D.H. Nguyen, B.K. Singh, H.B. Lee and E.K. Kim, Mol. Cell. Biochem., 403, 61 (2015).
S. J. Orlow and M. H. Brilliant, Exp. Eye. Res., 68, 2 (1999).
J.E. Hawkes, P.B. Cassidy, P. Manga, R.E. Boissy, D. Goldgar, L.C. Albright, S.R. Florell and S. A. Leachman, J. Dermatol. Sci., 69, (2013).
N. Puri, J. M. Gardner and M. H. Brilliant, J. Invest. Dermatol., 115 (2000).
S. Rosemblat, D. Durham-Pierre, J.M. Gardner, Y. Nakatsu, M.H. Brilliant and S. J. Orlow, Proc. Natl. Acad. Sci., 91 (1994).
P. Manga and S. J. Orlow, Pigment Cell Res., 14 (2001).
T. Suzuki and Y. Tomita, J. Dermatol. Sci., 51 (2008).
K. Chen, P. Manga and S. J. Orlow, Mol. Biol. Cell., 13 (2002).
K. Toyofuku, J. C. Valencia, T. Kushimoto, G. E. Costin, V. M. Virador and W.D. Vieira, Pigment Cell Res., 15 (2002).
D. L. Duffy, Z. Z. Zhao, R. A. Sturm, N. K. Hayward, N. G. Martin and G.W. Montgomery, J. Invest. Dermatol., 130 (2010).
K. E. Hummer and J. Jules, in Rosaceae: Taxonomy, Economic Importance, Genomics. In Genetics and Genomics of Rosaceae, Vol. 6 Plant Genetics and Genomics: Crops and Models, Ed. Kevin F and Susan G., Springer, New York (2009).
J. S. Choi, W. S. Woo, H. S. Young and J. H. Park, Arch. Pharm. Res., 13, 4 (1990).
J. S. Choi, H. S. Young, T.W. Lee, W. S. Woo and E. B. Lee, J. Pharmaceut. Soc. Korea, 1 (1992).
I. Shirasugi, Y. Sakakibara, M. Yamasaki, K. Nishiyama, T. Matsui, M.C. Liu and M. Suiko, Biosci. Biotechnol. Biochem., 74, 11 (2010).
L. H. Luo, H. J. Kim, D. H. Nguyen, H. B. Lee, N. H. Lee and E. K. Kim, Biol. Pharm. Bull., 32, 6 (2009).
G. Raposo and S.M. Michael, Nat. Rev. Mol. Cell. Bio., 8, 10 (2007).
S. Rosemblat, E.V. Sviderskaya, D. J. Easty, A. Wilson, B. S. Kwon, D.C. Bennett and S. J. Orlow, Exp. Cell Res., 239, 2 (1998).
H. B. Tamate, T. Hirobe, K. Wakamatsu, S. Ito, S. Shibahara and K. Ishikawa, J. Exp. Zool., 250 (1989).
G. Prota, M. L. Lamoreux, J. Muller, T. Kobayashi, A. Napolitano, M.R. Vincensi, C. Sakai and V. J. Hearing, Pigment Cell Res., 8 (1995).
T.R. Kramer and R. J. Noecker, Ophthalmol., 108, 4 (2001).
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Anti-melanogenic effect of Prunus davidiana extract in melan-a melanocyte through regulation of OCA-2, TRP-1 and tyrosinase
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Singh, B.K., Morya, V.K., Lee, HB. et al. Anti-melanogenic effect of Prunus davidiana extract in melan-a melanocyte through regulation of OCA-2, TRP-1 and tyrosinase. Korean J. Chem. Eng. 34, 3156–3162 (2017). https://doi.org/10.1007/s11814-017-0241-8
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DOI: https://doi.org/10.1007/s11814-017-0241-8