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Engineering a chimeric malate two-component system by introducing a positive feedback loop in Escherichia coli

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Abstract

Previous studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate concentration. The positive feedback loop was constructed by cloning ompR gene, which encodes ompC and induces OmpR protein; ompC promoter was used to control the process. The transcriptional expression profile showed that the expression level of ompC gene increased about two-fold after the positive feedback loop was introduced. When GFP was used as a reporter protein, a 71% increase in fluorescence level was observed. The results indicate that the signal transduction kinetics of MalKZ can be engineered by introducing the positive feedback loop.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Ulsan, Ulsan, 44610, Korea

    Irisappan Ganesh, Murali kannan Maruthamuthu & Soon Ho Hong

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  1. Irisappan Ganesh
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  2. Murali kannan Maruthamuthu
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  3. Soon Ho Hong
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Correspondence to Soon Ho Hong.

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Ganesh, I., Maruthamuthu, M.k. & Hong, S.H. Engineering a chimeric malate two-component system by introducing a positive feedback loop in Escherichia coli . Korean J. Chem. Eng. 33, 972–975 (2016). https://doi.org/10.1007/s11814-015-0209-5

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  • DOI: https://doi.org/10.1007/s11814-015-0209-5

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