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Chitosan-based bioglass composite for bone tissue healing : Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model

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Abstract

Tissue engineering has opened up a new therapeutic avenue promising a revolution in regenerative medicine. Considerable attention has been given to chitosan composite materials and their applications in the field of the bone graft substitutes. We evaluated the antioxidative properties of chitosan-doped bioactive glass (BG-CH) with 17 wt% chitosan, and their applications in the guided bone regeneration. BG-CH was produced by a freeze-drying process and implanted in the femoral condyles of ovariectomized rats. Grafted bone tissues were carefully removed to evaluate the oxidative stress analysis, histomorphometric profile and mineral bone distribution by using inductively coupled plasma optical emission spectrometry (ICP-OES). A significant decrease of thiobarbituric acid-reactive substances (TBARs) was observed after BG-CH implantation. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities significantly increased in ovariectomized group implanted with chitosan-doped bioactive glass (OVXBG-CH) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). The histomorphometric analysis showed that bone/tissue volume (BV/TV), osteoblast number (N.Ob) and osteoblast surface/bone surface (Ob.S/BS) were significantly higher in OVX-BG-CH group than in OVX-BG group. On the other hand, a rise in Ca and P ion concentrations in the implanted microenvironment was shown to lead to the formation/deposition of Ca-P phases. Trace elements such as Sr and Fe were detected in the newly formed bone and involved in bone healing. These results suggested that BG-CH composites could become clinically useful as a therapeutic and implantable material.

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Authors and Affiliations

  1. UMR CNRS 6226, University of Rennes 1, Campus de Beaulieu, 263 av. du Général Leclerc, 35042, Rennes, France

    Samira Jebahi & Hassane Oudadesse

  2. Animal Ecophysiology Laboratory, Sfax Faculty of Science, Department of Life Sciences, Sfax, Tunisia

    Samira Jebahi, Mongi Saoudi & Abdelfattah el Feki

  3. Science Materials and Environement Laboratory, Sfax Faculty of Science, Sfax, Tunisia

    Hafed el Feki

  4. Histology, Orthopaedic and Traumatology Laboratory Sfax Faculty of Medicine, Sfax, Tunisia

    Sirrar Mesadhi, Tarek Rebai & Hassib Keskes

  5. Laboratory of Human Molecular Genetics, Faculty of Medicine, University of Sfax, Sfax, Tunisia

    Gada Ben Saleh

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  1. Samira Jebahi
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  2. Hassane Oudadesse
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  3. Gada Ben Saleh
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  4. Mongi Saoudi
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Correspondence to Samira Jebahi.

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Jebahi, S., Oudadesse, H., Saleh, G.B. et al. Chitosan-based bioglass composite for bone tissue healing : Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model. Korean J. Chem. Eng. 31, 1616–1623 (2014). https://doi.org/10.1007/s11814-014-0072-9

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  • DOI: https://doi.org/10.1007/s11814-014-0072-9

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