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Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

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Abstract

Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.

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Author notes

  1. Eun Jeong Kim

    Present address: Biotechnology and Welfare R&D Coordination Division, Korea Institute of S&T Evaluation and Planning (KISTEP), 68, Mabang-gil, Seocho-gu, Seoul, 137-130, Korea

Authors and Affiliations

  1. Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, Daejeon, 305-340, Korea

    Mi-Ra Chang & Eun Jeong Kim

  2. Department of Dermatology, Seoul National University College of Medicine, 28, Yeongeon-dong, Jongno-gu, Seoul, 110-744, Korea

    Woong Hee Lee

  3. Division of Bioengineering, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon, 406-772, Korea

    Won Jong Rhee

  4. School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 151-744, Korea

    Tai Hyun Park

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  1. Mi-Ra Chang
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  2. Woong Hee Lee
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  3. Won Jong Rhee
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  4. Tai Hyun Park
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Correspondence to Tai Hyun Park.

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Chang, MR., Lee, W.H., Rhee, W.J. et al. Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages. Korean J. Chem. Eng. 30, 1784–1789 (2013). https://doi.org/10.1007/s11814-013-0108-6

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  • DOI: https://doi.org/10.1007/s11814-013-0108-6

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