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Fucoidan inhibits lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and zebrafish larvae

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Abstract

Fucoidan, a sulfated polysaccharide, is an active component found in various species of seaweed. Although this compound has a strong anti-inflammatory activity, the underlying mechanisms exerted by fucoidan have not been fully elucidated. In the present study, the anti-inflammatory effects of fucoidan on lipopolysaccharide (LPS)-stimulated macrophages and zebrafish larvae were examined. The present data indicated that fucoidan significantly suppressed the secretion of pro-inflammatory mediators including nitric oxide (NO ) and prostaglandin E2 (PGE2), and cytokines, such as tumor necrosis factor-α and interleukin-1β in RAW 264.7 macrophages without any significant cytotoxicity, the protective effects of which were accompanied by a marked reduction in their regulatory gene expression at the transcription levels. Fucoidan also inhibited translocation of the nuclear factor-kappa B from the cytoplasm to the nucleus and attenuated LPS-induced production of intracellular reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, fucoidan reduced NO and PGE2 production and ROS accumulation in LPS-stimulated zebrafish larvae, which was associated with a diminished recruitment of neutrophils and macrophages. Based on the results of this study, we suggest that fucoidan has excellent potential as a therapeutic agent for inflammatory disorders.

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Authors and Affiliations

  1. Anti-Aging Research Center, Dongeui University, Busan, 47340, Republic of Korea

    Jin-Woo Jeong & Yung Hyun Choi

  2. Department of Biochemistry, Dongeui University College of Korean Medicine, Busan, 47227, Republic of Korea

    Jin-Woo Jeong & Yung Hyun Choi

  3. Department of Pharmacy, College of Pharmacy, Inje University, Gimhae, 50834, Republic of Korea

    Su Jung Hwang & Hyo-Jong Lee

  4. Natural products Research Team, Marine Biodiversity Institute of Korea, Seocheon, 33662, Republic of Korea

    Min Ho Han, Dae-Sung Lee & Jong Su Yoo

  5. Department of Microbiology, College of Medicine, Inje University, Busan, 47392, Republic of Korea

    Il-Whan Choi

  6. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, 49267, Republic of Korea

    Hee-Jae Cha

  7. Department of Chemistry, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea

    Suhkmann Kim

  8. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Republic of Korea

    Heui-Soo Kim

  9. Department of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of Korea

    Gi-Young Kim & You-Jin Jeon

  10. Department of Physiology, Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, Busan, 49201, Republic of Korea

    Hwan Tae Park

  11. Department of Anatomy and Cell Biology, Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, Busan, 49201, Republic of Korea

    Young Hyun Yoo

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  1. Jin-Woo Jeong
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Correspondence to Young Hyun Yoo or Yung Hyun Choi.

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Jeong, JW., Hwang, S.J., Han, M.H. et al. Fucoidan inhibits lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and zebrafish larvae. Mol. Cell. Toxicol. 13, 405–417 (2017). https://doi.org/10.1007/s13273-017-0045-2

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  • DOI: https://doi.org/10.1007/s13273-017-0045-2

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