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Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion

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Abstract

Hydrothermal fractionation for micro-algae, Schizocytrium sp., was investigated to separate sugars, lipids, and proteins. This fractionation process produced protein-rich solid cake and liquid hydrolysates, which contained oligomeric sugars and lipids. Oligomeric sugars and lipids were easily separated by liquid-liquid separation. Sugars in the separated hydrolyzate were determined to be mainly D-glucose and L-galactose. Fractionation conditions were optimized by response surface methodology (RSM). Optimal conditions were found to be 115.5 °C of reaction temperature, 46.7 min of reaction time, and 25% (w/w) of solid loading. The model predicted that maximum oligomeric sugar yield (based on untreated micro-algae weight), which can be recovered by hydrothermal fractionation at the optimum conditions, was 19.4 wt% (based on the total biomass weight). Experimental results were in agreement with the model prediction of 16.6 wt%. Production of bioethanol using micro-algae-induced glucan and E. coli KO11 was tested with SSF (simultaneous saccharification and fermentation), which resulted in 11.8 g-ethanol/l was produced from 25.7 g/l of glucose; i.e. the theoretical maximum ethanol yield based on glucan in hydrolyzate was 89.8%.

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Authors and Affiliations

  1. Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Jeonnam, 534-833, Korea

    Jung Kon Kim

  2. Division of Chemical Engineering, Hankyong National University, Anseong, Gyeonggi-do, 456-749, Korea

    Byung-Hwan Um

  3. Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, 50011, USA

    Tae Hyun Kim

  4. Department of Natural Resources Ecology and Management, Iowa State University, Ames, IA, 50011, USA

    Tae Hyun Kim

  5. Center for Crops Utilization and Research, Iowa State University, Ames, IA, 50011, USA

    Tae Hyun Kim

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  1. Jung Kon Kim
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  2. Byung-Hwan Um
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Kim, J.K., Um, BH. & Kim, T.H. Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion. Korean J. Chem. Eng. 29, 209–214 (2012). https://doi.org/10.1007/s11814-011-0169-3

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  • DOI: https://doi.org/10.1007/s11814-011-0169-3

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